Render Target: SSR
Render Timestamp: 2025-03-16T00:09:44.052Z
Commit: a619ae74f66dae0f27639e88da12bcf600e46428
XML generation date: 2025-03-07 13:12:41.619
Product last modified at: 2025-01-01T09:01:18.983Z
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PDP - Template Name: Growth Factors and Cytokines
PDP - Template ID: *******9ad1159

Platelet-Derived Growth Factor (PDGF) #9909

Inquiry Info. # 9909

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    Product Information

    Product Usage Information

    CST recommends using 50-100 ng/ml of PDGF for stimulation of PDGF signaling.

    Storage

    Platelet-Derived Growth Factor (PDGF) is supplied as a solution. It should be stored at –80°C. Aliquot the reagent upon receipt and avoid repeated freeze-thaw cycles.

    Product Description

    The human PDGF-B coding cDNA was obtained from hepatocytic mRNA, subcloned into an expression vector and expressed in yeast. The recombinant human PDGF-BB dimer was purified and stored in PBS buffer containing 0.1% BSA.
    MW (kDa) 29
    Purity >97%
    Activity 1 x 105 IU/mg

    Source / Purification

    Human Recombinant Protein

    Background

    Platelet-derived growth factor (PDGF) is a dimeric molecule that exists in homodimers or heterodimers of related polypeptide chains (A and B). Two types of PDGF receptors have been identified. The PDGF alpha-receptor binds all three PDGF isoforms with high affinity, whereas the beta-receptor binds only PDGF-BB with high affinity, PDGF-AB with low affinity, and does not appear to bind PDGF-AA (1). Ligand binding induces receptor dimerization and autophosphorylation, allowing binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules. A number of different signaling pathways are then initiated, leading to cell growth, actin reorganization, migration, and differentiation (2-4). In clinical studies, PDGF expression has been demonstrated in a number of different solid tumors, from glioblastomas to prostate carcinomas. In these various tumor types, the biological role of PDGF signaling can vary from autocrine stimulation of cancer cell growth to more subtle paracrine interactions involving adjacent stroma and even angiogenesis. Targeting PDGF signaling is becoming an effective tumor treatment method (5).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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