Mouse IL-1α Recombinant Protein #88205
Product Information
Storage
Mouse IL-1α Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/ml which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.
Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.
Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.
Product Description
| MW (kDa) | 18.1 |
| Purity | A greater than or equal to 95% purity was determined by SDS-PAGE. |
| Endotoxin | Endotoxin levels are less than or equal to 1 EU / 1 μg mIL-1α. |
| Activity | The bioactivity of recombinant mIL-1α was determined in a D10.G4.1 cell proliferation assay. The ED50 of each lot is less than or equal to 10 pg/ml. |
Source / Purification
Recombinant mouse IL-1α was expressed in E. coli and is supplied in a lyophilized form.
Background
Interleukin 1 alpha (IL-1α) belongs to the IL-1 family of cytokines with 11 members including IL-1β. IL-1α is expressed in many cell types of both hematopoietic and non-hematopoietic origins under steady state, and its expression can be increased in response to appropriate stimuli (1,2). Like IL-1β, IL-1α is also synthesized as a precursor (pro-IL-1α) and can be cleaved into smaller mature forms. However, both pro-IL-1α and the cleaved form of IL-1α are biologically active and can activate the signaling pathway through the membrane receptor IL-1R1. IL-1α is active both as a secreted form and as a membrane-bound form. Due to such characteristics, passive leakage of IL-1α from dying cells can activate inflammation, leading some researchers to consider IL-1α as a key “alarmin in the cell” that alerts the host to damage or injury (3,4). In addition, IL-1α can also enter the nucleus to modulate transcription (5,6).
- Garlanda, C. et al. (2013) Immunity 39, 1003-18.
- Palomo, J. et al. (2015) Cytokine 76, 25-37.
- Bertheloot, D. and Latz, E. (2017) Cell Mol Immunol 14, 43-64.
- Di Paolo, N.C. and Shayakhmetov, D.M. (2016) Nat Immunol 17, 906-13.
- Lamacchia, C. et al. (2013) Cytokine 63, 135-44.
- Rider, P. et al. (2013) Semin Immunol 25, 430-8.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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