Human VEGF-121 Recombinant Protein #38285
Product Information
Storage
Human VEGF-121 Recombinant Protein is supplied as lyophilized material that is very stable at -20ºC. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/mL which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.
Once in storage, store at 4ºC and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.
Once in storage, store at 4ºC and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.
Product Description
| MW (kDa) | 14.2/28.4 |
| Purity | A greater than or equal to 95% purity was determined by SDS-PAGE. |
| Endotoxin | Endotoxin levels are less than or equal to 0.1 EU / 1 μg hVEGF-121. |
| Activity | The bioactivity of recombinant hVEGF-121 was determined in a HUVEC proliferation assay. The ED50 of each lot is less than or equal to 5 ng/mL. |
Source / Purification
Recombinant human VEGF-121 was expressed in E. coli and is supplied in a lyophilized form.
Background
VEGF121 is the second most abundant splice variant of vascular endothelial growth factor-A (VEGF-A; formerly VEGF) (1,2). VEGF121 is produced by endothelial cells, macrophages, T cells, and other cell types. VEGF121 is involved in angiogenesis, vascular endothelial cell survival, growth, migration, and vascular permeability (1). VEGF121 expression is induced by hypoxia, inflammatory cytokines, and through oncogene products in tumors (1-3). VEGF121 binds to VEGFR1 and VEGFR2 receptor tyrosine kinases (1). Binding of VEGF121 to VEGFR1 and VEGFR2 leads to activation of pathways involving PI3K/Akt, p38 MAPK, and FAK (1). VEGF plays a key role in tumor angiogenesis in many cancers (2).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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