Human Stem Cell Factor (SCF) #9907
Inquiry Info. # 9907
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Product Information
Product Usage Information
CST recommends using 50-100 ng/ml of SCF for stimulation of SCF signaling.
Storage
Human SCF is supplied as a solution. It should be stored at –80°C. Aliquot the reagent upon receipt and avoid repeat freeze-thaw cycles.
Product Description
The human SCF coding cDNA was obtained from human bone marrow stromal cell mRNA, subcloned into a prokaryotic expression vector and expressed in E. coli. The recombinant human SCF protein was purified and stored in PBS buffer containing 0.1% BSA.
| MW (kDa) | 18.5 |
| Purity | >97% |
| Activity | The EC50 for stimulation of TF-1 cell proliferation is 4-8 ng/ml. |
Source / Purification
Human Recombinant Protein
Background
Stem cell factor (SCF) is a growth factor that is essential for hematopoiesis, melanogenesis and fertility. SCF is also known as mast cell growth factor (MCGF), steel factor (SLF), or kit ligand (KL) (1-3). SCF mediates its biological effects by binding to and activating c-Kit (4). SCF induces dimerization of c-Kit followed by trans-autophosphorylation of the cytoplasmic protein tyrosine kinase domain, leading to subsequent recruitment of signaling proteins, tyrosine phosphorylation of substrates, and activation of multiple signaling pathways (5,6). SCF/c-Kit may take part in the growth control of human malignancies (7).
- Smith, M.A. et al. (2001) Blood Rev. 15, 191-197.
- Linnekin, D. (1999) Int. J. Biochem. Cell Biol. 31, 1053-1074.
- Huang, E. et al. (1990) Cell 63, 225-233.
- Jiang, X. et al. (2000) EMBO J. 19, 3192-3203.
- Ashman, L.K. (1999) Int. J. Biochem. Cell Biol. 31, 1037-1051.
- Ueda, S. et al. (2002) Blood 99, 3342-3349.
- Attoub, S. et al. (2002) Cancer Res. 62, 4879-4883.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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