Human/Mouse TGF-β3 Recombinant Protein #10858
Product Information
Storage
Human/Mouse TGF-β3 Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/mL which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.
Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.
Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.
Product Description
| MW (kDa) | 25.8 |
| Purity | A greater than or equal to 95% purity was determined by SDS-PAGE. |
| Endotoxin | Endotoxin levels are less than or equal to 1 EU / 1 μg h/mTGF-β. |
| Activity | The bioactivity of recombinant h/mTGF-β3 was determined in an IL-4 induced HT-2 cell proliferation assay. The ED50 of each lot is less than or equal to 0.038 ng/mL. |
Source / Purification
Recombinant human/mouse TGF-β3 was expressed in E. coli and is supplied in a lyophilized form.
Background
TGF-β3 can induce cell proliferation and angiogenesis, and can promote some immune events while inhibiting others (1-3). TGF-β3 is produced by chondrocytes, glial cells, astrocytes, and some cancer cells (3,4). TGF-β3 binds to TβRII homodimer, which then complexes with TβRI homodimer (1,5). The oligomeric receptor complex phosphorylates subsets of SMAD proteins that then act to induce or suppress target genes (1,5). Based on knockout studies in mice, TGF-β3 is critical for lung and palate development (6).
- Siegel, P.M. and Massagué, J. (2003) Nat Rev Cancer 3, 807-21.
- Bierie, B. and Moses, H.L. (2006) Nat Rev Cancer 6, 506-20.
- Damstrup, L. et al. (1993) Br J Cancer 67, 1015-21.
- Constam, D.B. et al. (1992) J Immunol 148, 1404-10.
- Moustakas, A. and Heldin, C.H. (2009) Development 136, 3699-714.
- Dünker, N. and Krieglstein, K. (2000) Eur J Biochem 267, 6982-8.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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