Human Interleukin-21 (hIL-21) Recombinant Protein #45095
Product Information
Product Usage Information
Working concentration of recombinant hIL-21 generally ranges from 0.1-10 ng/ml.
Storage
Recombinant human IL-21 is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/ml which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long term storage.
Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.
Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.
Product Description
| MW (kDa) | 16 |
| Purity | A greater than 95% purity was determined by SDS-PAGE. |
| Endotoxin | Less than or equal to 1 EU / 1 μg hIL-21. |
| Activity | The bioactivity of recombinant hIL-21 was determined by its ability to induce IFN-γ production in B9 cells. The ED50 of each lot is between 25-50 ng/ml. |
Source / Purification
Recombinant human IL-21 was expressed in E. coli and is supplied in a lyophilized form.
Background
IL-21 is produced by Th17, T follicular helper (Tfh) and NKT cells (1-3). Targets include T cells, B cells, NK cells and dendritic cells (1-3,5). IL-21 induces proliferation and activation of NK cells, thereby up-regulating IFN-γ production and cytotoxic activity (1,3,4). IL-21 increases proliferation and survival of CD40-primed B cells (1,2) and appears to have a significant role in plasma cell differentiation (2). IL-21 binds to a complex consisting of IL-21Rα and the common γ chain, γc. IL-21 binding activates the Jak1/Jak3, Stat1, Stat3 and Stat5 pathway. IL-21 binding can also activate the MAP kinase and PI3K/Akt pathways.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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