Human IL-5 Recombinant Protein #44227
Product Information
Storage
Human IL-5 Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/ml which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long term storage.
Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.
Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.
Product Description
| MW (kDa) | 26 |
| Purity | A greater than or equal to 95% purity was determined by SDS-PAGE. |
| Endotoxin | Endotoxin levels are less than or equal to 1 EU / 1 μg hIL-5. |
| Activity | The bioactivity of recombinant hIL-5 was determined in a TF-1 cell proliferation assay. The ED50 of each lot is less than or equal to 250 pg/ml. |
Source / Purification
Recombinant human IL-5 was expressed in E. coli and is supplied in a lyophilized form.
Background
IL-5 is a pleiotropic cytokine that is predominantly produced by TH2 T cells but can also be expressed by activated eosinophils, mast cells, NK cells, and iNKT cells (1-4). Both human and mouse IL-5 are glycosylated disulfide-linked homodimers (1). The IL-5 receptor is a heterodimer that consists of a high affinity IL-5 binding α chain and the common β chain, which is shared by GM-CSF and IL-3 receptors, for signal transduction (1,2). Soluble IL-5Rα binds with high affinity to IL-5, thereby inhibiting IL-5 activity (1). IL-5-mediated signaling can activate the Erk1/2, Jak2, and Stat5 signaling pathways (1,2). In mice, IL-5 is important for the differentiation of antibody-secreting cells from activated B cells (2). IL-5 induces eosinophil activation, proliferation, and differentiation in both mice and humans (2,3).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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