Human IL-3 Recombinant Protein #36940
The purity of Human IL-3 Recombinant Protein was determined by SDS-PAGE of 1.5 µg non-reduced (-) and reduced (+) recombinant hIL-3 and staining with Coomassie Blue.
Product Information
Storage
Human IL-3 Recombinant Protein is supplied as a lyophilized material that is very stable at -20ºC. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/mL which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.
Once in solution, store at 4ºC and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.
Once in solution, store at 4ºC and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.
Product Description
| MW (kDa) | 13-16 |
| Purity | A greater than 95% purity was determined by SDS-PAGE. |
| Endotoxin | Endotoxin levels are less than 1 EU/ 1 µg hIL-3. |
| Activity | The bioactivity of recombinant IL-3 was measured by human TF-1 erythroleukemic cells. The ED50 is between 0.05-0.3 ng/mL. |
Source / Purification
Recombinant human Interleukin-3 was expressed in E.coli and is supplied in a lyophilized form.
Background
Interleukin-3 (IL-3) is produced by T cells, mast cells, and eosinophils (1). Target cells include hematopoietic progenitors, neutrophils, macrophages, mast cells, eosinophils, lymphoid, and erythroid cells (1). The IL-3 receptor is a heterodimer of the IL-3 specific α-chain and the common β-chain, βc, which is also used by GM-CSF and IL-5. (1). Binding of IL-3 can also involve substitution of the βc by a βIL-3-chain that appears to be specific for IL-3 (1,2). Binding of IL-3 to its cognate receptor(s) induces activation of Jak2, phosphorylation of multiple Stats (1,3,5,6), and the PI3K/Akt pathway (1). IL-3 may play an important role in the development of airway inflammation associated with asthma (3-5).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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