Human IL-18 Recombinant Protein #15456
Product Information
Storage
Human IL-18 Recombinant Protein is supplied as lyophilized material that is very stable at -20ºC. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/mL which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.
Once in solution, store at 4ºC and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.
Once in solution, store at 4ºC and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.
Product Description
| MW (kDa) | 17-19 |
| Purity | A greater than 95% purity was determined by SDS-PAGE. |
| Endotoxin | Endotoxin levels are less than 1 EU / 1 μg hIL-18. |
| Activity | The bioactivity of recombinant hIL-18 was determined in a KG-1 cell proliferation assay by measuring the induction of IFN-γ secretion in the presence of TNF-α. The ED50 of each lot is less than or equal to 15.26 ng/mL in the presence of 15 ng/mL TNF-α. |
Source / Purification
Recombinant human IL-18 was expressed in E. coli and is supplied in a lyophilized form.
Background
Interleukin-18 (IL-18), originally known as interferon-gamma inducing factor (IGIF), is part of the IL-superfamily of cytokines (1,2). This proinflammatory cytokine is synthesized as an inactive precursor which requires cleavage by Caspase-1 to become an active, mature molecule, which is secreted by monocytes and macrophages (3,4,6). It induces IFNγ production in T-helper-1 (Th1) cells and natural killer (NK) cells, and can regulate innate immunity via both Th1 and Th2 responses (4,5). Elevated IL-18 levels are associated with autoimmune disease, but may be balanced by binding to IL-18 binding protein (IL-18BP), which along with IL-18 neutralizing antibodies, are being examined in clinical trials (6,7).
- Okamura, H. et al. (1995) Nature 378, 88-91.
- Ushio, S. et al. (1996) J Immunol 156, 4274-9.
- Ghayur, T. et al. (1997) Nature 386, 619-23.
- Dinarello, C.A. (1999) Methods 19, 121-32.
- Nakanishi, K. et al. (2001) Annu Rev Immunol 19, 423-74.
- Novick, D. et al. (1999) Immunity 10, 127-36.
- Dinarello, C.A. et al. (2013) Front Immunol 4, 289.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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