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Render Timestamp:
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3/27/2025, 5:56:58 PM UTC
Commit: 461ca8d8fe5b1efd4c01fc87e5b5eb592e2d154a
XML generation date: 2025-03-11 22:02:16.009
Product last modified at: 2025-03-13T08:00:11.756Z
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PDP - Template Name: Growth Factors and Cytokines
PDP - Template ID: *******9ad1159

Human IL-17A Recombinant Protein #90536

    Product Information

    Storage

    Human IL-17A Recombinant Protein is supplied as lyophilized material that is very stable at -20ºC. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/mL which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.

    Once in solution, store at 4ºC and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.

    Product Description

    MW (kDa) 15.7/31.3
    Purity A greater than or equal to 95% purity was determined by SDS-PAGE.
    Endotoxin Endotoxin levels are less than or equal to 1 EU / 1 μg hIL-17A.
    Activity The bioactivity of recombinant human IL-17A (hIL-17A) was determined by production of IL-6 from a mouse NIH/3T3 cell proliferation assay. The ED50 of each lot is less than or equal to 10 ng/mL.

    Source / Purification

    Recombinant hIL-17A was expressed in E. coli and is supplied in a lyophilized form.

    Background

    Interleukin-17A (IL-17A) is a cystine-linked homodimeric pro-inflammatory cytokine produced by Th17 cells, a distinct CD4+ T cell lineage (1,2). IL-17A stimulates production of the pro-inflammatory cytokines IL-1β, TNF-α, and IL-6. IL-17A also induces production of the neutrophil chemoattractants IL-8, CXCL1, and CXCL6 thereby bridging adaptive and innate immunity (1,2). IL-17A is intimately involved in mucosal immunity against bacterial infections (1,3) and has a putative role in some autoimmune disorders (1,4). IL-17A effects appear to be exerted primarily through binding to IL-17RA (5). IL-17A binding induces production of cytokines, chemokines, and other proteins through activation of the Erk1/2 MAP kinase, PI3K/Akt, p38, and NF-κB pathways (3,4,6). Phosphorylation of some Jaks and Stats has been observed.
    For Research Use Only. Not For Use In Diagnostic Procedures.
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