Human IL-17A/F Heterodimer Recombinant Protein #64210
The purity of Human IL-17A/F Heterodimer Recombinant Protein was determined by SDS-PAGE of 1 µg reduced (+) and non-reduced (-) recombinant hIL-17A/F and staining with Coomassie Blue. hIL-17A/F is a heterodimer with a predicted total molecular weight (MW) of 30.7 kDa with the IL-17A monomer equaling 15.7 kDa and the IL-17F monomer equaling 15.0 kDa.
Product Information
Storage
Human IL-17A/F Heterodimer Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/mL which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.
Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.
Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.
Product Description
| MW (kDa) | 30.7 |
| Purity | A greater than or equal to 95% purity was determined by SDS-PAGE. |
| Endotoxin | Endotoxin levels are less than or equal to 1 EU / 1 μg hIL-17A/F. |
| Activity | The bioactivity of recombinant human IL-17A/F was determined by measuring the production of IL-6 from mouse 3T3 cells. The ED50 of each lot is less than or equal to 10 pg/mL. |
Source / Purification
Recombinant human IL-17A/F was expressed in E. coli and is supplied in a lyophilized form.
Background
IL-17A/F is a cystine-linked heterodimer of IL-17A and IL-17F (1,2). IL-17A/F is produced by Th17 cells and γδ T cells (1,2). IL-17A/F stimulates production of pro-inflammatory cytokines and neutrophil chemoattractants, thereby functioning as a bridge between adaptive and innate immunity (1-3). Some research studies suggest that IL-17A/F may be involved in mucosal immunity against some bacterial infections and has a putative role in some autoimmune disorders (3,4). The receptor for IL-17A/F consists of a heterodimer of IL-17RA and IL-17RC and signaling through this receptor leads to activation of the Erk1/2 and NF-κB pathways (1).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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