Human His6FasL/TNFSF6 Recombinant Protein #53789
Product Information
Storage
Animal-free Human His6FasL/TNFSF6 Recombinant Protein is supplied as a lyophilized material that is very stable at -20ºC. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/mL which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.
Once in solution, store at 4ºC and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.
Once in solution, store at 4ºC and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.
Product Description
| MW (kDa) | 17.3 |
| Purity | A greater than 95% purity was determined by SDS-PAGE. |
| Endotoxin | Endotoxin levels are less than or equal to 1 EU/1 µg hHis6FasL/TNFSF6. |
| Activity | The bioactivity of recombinant human His6FasL/TNFSF6 was measured by its ability to induce apoptosis in Jurkat cells. The ED50 for this effect is <1 ng/mL. |
Source / Purification
Animal-free recombinant human His6FasL/TNFSF6 was expressed in E. coli with an N-terminal His-tag and is supplied in a lyophilized form.
Background
FasL is a member of the TNF superfamily of proteins and is expressed primarily on the cell surface of activated T and NK cells (1). FasL regulates the immune response through its ability to induce apoptosis. The immunoregulatory role of FasL is underscored by lymphadenopathy associated with FasL or Fas knockout mice and the fraction of autoimmune lymphoproliferative syndrome (ALPS) patients that have mutations in the FasL receptor, Fas (1). FasL is a membrane protein that can be cleaved into a soluble trimeric form by metalloproteinases (1,2). The soluble form of FasL retains the ability to bind to Fas, however, its ability to induce apoptosis is diminished (2). The ligation of Fas by FasL leads to the assembly of death-inducing signaling complex (DISC) and the recruitment and activation of caspase-8/caspase-10 (1,3). Active caspase-8/caspase-10 subsequently activates the “effector” caspases caspase-3 and caspase-7, and cleavage of BID (1,3).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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