Human His641BB Ligand/TNFSF9 (hHis64-1BBL) #8460
Inquiry Info. # 8460
Please see our recommended alternatives.
Product Information
Formulation
With carrier: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2 containing 20 μg BSA per 1 μg hHis64-1BBL. Carrier free: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.2.
Storage
Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted with carrier protein are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.
Maintain sterility. Storage at -20°C should be in a manual defrost freezer.
Maintain sterility. Storage at -20°C should be in a manual defrost freezer.
Product Description
| MW (kDa) | 22 |
| Purity | >98% as determined by SDS-PAGE of 6 μg reduced (+) and non-reduced (-) recombinant hHis64-1BBL. All lots are greater than 98% pure. |
| Endotoxin | Less than 0.01 ng endotoxin/1 μg hHis64-1BBL. |
| Activity | The activity of hHis64-1BBL was assessed by its ability to bind to 4-1BB in a functional ELISA. The concentration at which half-maximal binding was observed for each lot was 6-18 ng/ml. |
| Molecular Formula | Recombinant N-terminally His6-tagged h4-1BBL has a calculated MW of 22,020. DTT-reduced and non-reduced protein migrate as 22 kDa polypeptides. The expected amino terminus of recombinant hHis64-1BBL was verified by amino acid sequencing. |
Source / Purification
Recombinant human His64-1BBL (hHis64-1BBL) Arg71-Glu254 (Accession #NP_003802) was expressed in human 293 cells at Cell Signaling Technology.
Background
4-1BBL (also known as TNFSF9) is a member of the TNF-R superfamily, which includes OX40L, CD27L, CD40L, and GITRL (1). 4-1BBL is expressed primarily on antigen presenting cells, such as macrophages, dendritic cells, and B cells (2). 4-1BBL binds to 4-1BB on the surface of activated T cells (1). 4-1BBL/4-1BB binding activates the NF-κB pathway via TRAF1, 2, and 3 (1). 4-1BBL functions as a co-stimulatory molecule, inducing T cell proliferation, cytolytic activity, and cytokine production (2). Matrix metalloproteases can generate soluble 4-1BBL by cleaving it from the cell surface (3). The in vitro activity of soluble 4-1BBL is enhanced by cross-linking (4,5).
限制使用
除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。
专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our
Trademark Information page.