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Render Timestamp: 2024-12-10T21:31:15.253Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-09-20 06:23:37.568
Product last modified at: 2024-05-30T07:03:03.117Z
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PDP - Template Name: Growth Factors and Cytokines
PDP - Template ID: *******9ad1159

Human G-CSF Recombinant Protein #48713

    Product Information

    Storage

    Humant G-CSF Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/mL which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage.

    Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.

    Product Description

    MW (kDa) 18.8
    Purity A greater than or equal to 95% purity was determined by SDS-PAGE.
    Endotoxin Endotoxin levels are less than or equal to 1 EU / 1 μg hG-CSF.
    Activity The bioactivity of recombinant hG-CSF was determined in an M-NFS-60 cell proliferation assay. The ED50 of each lot is less than or equal to 60 pg/mL.

    Source / Purification

    Recombinant human G-CSF was expressed in E. coli and is supplied in a lyophilized form.

    Background

    G-CSF is a hematopoietic cytokine essential for neutrophil development, survival, and egress from bone marrow (1-4). Macrophages and monocytes are the predominant producers of G-CSF (3) and endothelial cells, fibroblasts, and neuronal cells can produce G-CSF in response to inflammatory stimuli (3). G-CSF inhibits apoptosis in neutrophils and neurons (4,5). G-CSF stimulates proliferation and differentiation of neuronal progenitor cells (5). G-CSF binding to G-CSFR induces receptor dimerization and activation of Jak1/2 tyrosine phosphorylation (3,6). Signaling is through Stat3, ERK, p38, and Akt (5,6). Absence of functional G-CSF or its receptor in humans and mice causes neutropenia (7,8).
    For Research Use Only. Not For Use In Diagnostic Procedures.
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