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Render Timestamp: 2024-11-14T22:31:28.193Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-09-20 06:17:47.042
Product last modified at: 2024-08-23T18:30:09.564Z
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PDP - Template Name: Growth Factors and Cytokines
PDP - Template ID: *******9ad1159

Human FGF-basic/FGF2 (154 aa) Recombinant Protein #61977

    Product Information

    Product Usage Information

    Working concentration of hFGF-basic generally ranges from 0.1-10 ng/ml.

    Storage

    Human FGF-basic Recombinant Protein is supplied as lyophilized material that is very stable at -20°C. It is recommended to reconstitute with sterile water at a concentration of 0.1 mg/ml which can be further diluted in aqueous solutions as needed. Addition of a carrier protein (0.1% HSA or BSA) is recommended for long term storage.

    Once in solution, store at 4°C and use within 1 month, or store at -20ºC to -80ºC and use within 3 months to prevent loss of potency. Aliquot to avoid multiple freeze/thaw cycles if storing reconstituted material at -20ºC to -80ºC.

    Product Description

    MW (kDa) 17
    Purity A greater than 95% purity was determined by SDS-PAGE.
    Endotoxin Less than or equal to 1 EU / 1 μg hFGF-basic
    Activity The bioactivity of Human FGF-basic Recombinant Protein was determined in a NIH/3T3 cell proliferation assay. The ED50 of each lot is between 0.05 - 1.0 ng/ml.

    Source / Purification

    Human FGF-basic Recombinant Protein was expressed in E. coli and is supplied in a lyophilized form.

    Background

    FGF basic (FGF2) is produced by epithelial, tumor and other cell types (1). FGF basic is involved in developmental processes and regulates differentiation, proliferation, and migration (1-6). FGF basic is a critical factor for growing embryonic stem cells in culture without inducing differentiation. FGF basic has a high affinity for heparan sulfate (1,2) and FGF-heparan sulfate binding is a step in the activation of FGFR tyrosine kinase. There are four distinct FGF receptors and each has multiple splice variants. FGF basic binds with high affinity to many, but not all, FGFRs. Signaling cascades activated through FGF basic binding to FGFR include the ras-raf-MAPK, PLCγ/PKC, and PI3K/AKT pathways (1).
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