Render Target: SSR
Render Timestamp: 2024-12-10T21:30:47.756Z
Commit: 611277b6de3cd1bb065350b6ef8d63df412b7185
XML generation date: 2024-09-20 06:14:08.463
Product last modified at: 2024-11-28T07:00:09.194Z
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Micrococcal Nuclease #10011

    Product Information

    Product Usage Information

    Use during chromatin fragmentation in chromatin immunoprecipitation (ChIP) assays as directed in the protocols for the SimpleChIP® and SimpleChIP® Plus Enzymatic Chromatin IP Kits. For proper chromatin digestion, micrococcal nuclease requires the use of SimpleChIP® Lysis Buffers A & B #14282.

    Storage

    Supplied in 10 mM Tris-HCl (pH 7.5), 50 mM NaCl, 1mM EDTA and 50% glycerol. Store at –20°C. Do not aliquot the product. This product is stable for 12 months.

    Product Description

    Micrococcal nuclease is a relatively non-specific endo-exonuclease derived from Staphylococcus aureus. Active in the pH range of 7.0-10.0, this product digests double-stranded, single-stranded, circular, and linear nucleic acids.

    In the SimpleChIP® Enzymatic Chromatin IP kit assay, following cell lysis, the chromatin is fragmented by partial digestion with micrococcal nuclease to obtain chromatin fragments of 1 to 5 nucleosomes in size. Enzymatic digestion of chromatin is much milder than sonication and eliminates problems due to variability in sonication power and emulsification of chromatin during sonication, which can result in incomplete fragmentation of chromatin or loss of antibody epitopes due to protein denaturation and degradation.

    This product is offered to conveniently provide additional micrococcal nuclease when fragmenting chromatin with our SimpleChIP® (#9002, #9003) and SimpleChIP® Plus (#9004, #9005) Enzymatic Chromatin IP Kits. These kits provide all the reagents required for performing 6 chromatin preparations (or optimizations) and 30 chromatin immunoprecipitation (ChIP) assays, however there are instances where extra micrococcal nuclease is desired.
    For Research Use Only. Not For Use In Diagnostic Procedures.
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