DNA Library Prep Kit for Illumina Systems (ChIP-seq, CUT&RUN) #56795
- ChIP
- C&R
Product Information
Storage
Protocol
Product Description
Next generation sequencing (NG-seq) is a high throughput method that can be used downstream of chromatin immunoprecipitation (ChIP) and Cleavage Under Targets and Release Using Nuclease (CUT&RUN) assays to identify and quantify target DNA enrichment across the entire genome. The DNA Library Prep Kit for Illumina Systems (ChIP-seq, CUT&RUN) contains all of the enzymes and buffers necessary to generate high quality DNA sequencing libraries from ChIP or CUT&RUN DNA for next-generation sequencing on the Illumina Systems platform. The fast, user-friendly workflow minimizes hands-on time needed for generation and purification of DNA libraries. This product must be used in combination with Multiplex Oligos for Illumina Systems (Single Index Primers) (ChIP-seq, CUT&RUN) #29580 or Multiplex Oligos for Illumina Systems (Dual Index Primers) (ChIP-seq, CUT&RUN) #47538.
This product provides sufficient amounts of reagents for 24 reactions and is compatible with both enzymatic- or sonication-fragmented, ChIP-enriched DNA. Distinct protocols are provied for DNA library preparation from ChIP and CUT&RUN DNA. This product is compatible with SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003, SimpleChIP® Plus Enzymatic Chromatin IP Kit (Magnetic Beads) #9005, SimpleChIP® Plus Sonication Chromatin IP Kit #56383, and CUT&RUN Assay Kit #86652. This product is not compatible with SimpleChIP® Enzymatic Chromatin IP Kit (Agarose Beads) #9002 and SimpleChIP® Plus Enzymatic Chromatin IP Kit (Agarose Beads) #9004 because agarose beads are blocked with sonicated salmon sperm DNA, which will contaminate DNA library preps and NG-seq.
Specificity / Sensitivity
Species Reactivity:
Background
- Orlando, V. (2000) Trends Biochem Sci 25, 99-104.
- Kuo, M.H. and Allis, C.D. (1999) Methods 19, 425-33.
- Agalioti, T. et al. (2000) Cell 103, 667-78.
- Soutoglou, E. and Talianidis, I. (2002) Science 295, 1901-4.
- Mikkelsen, T.S. et al. (2007) Nature 448, 553-60.
- Lee, T.I. et al. (2006) Cell 125, 301-13.
- Weinmann, A.S. and Farnham, P.J. (2002) Methods 26, 37-47.
- Wells, J. and Farnham, P.J. (2002) Methods 26, 48-56.
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