TUNEL Assay Kit (Fluorescence, 488 nm) #25879
Confocal analysis of paraffin-embedded HT-29 xenograft using TUNEL Assay Kit (Fluorescence, 488 nm) (green) and DAPI #4083 (blue).
- Product Includes
- Related Products
| Product Includes | Quantity (with Count) |
|---|---|
| TUNEL Assay Equilibration Buffer #84862 | 1 x 5 ml |
| CF® 488 TUNEL Reaction Buffer #39670 | 5 x 500 µl |
| TdT Enzyme #79533 | 1 x 50 µl |
Product Information
Protocol
Product Description
TUNEL (terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling) is a method by which a fluorophore conjugated nucleotide is enzymatically linked to the 3′ end of fragmented DNA. Since DNA fragmentation is a hallmark of apoptosis, the TUNEL assay has become a well established method to monitor apoptosis in situ. The TUNEL Assay Kit (Fluorescence, 488 nm) provides you with the buffers, enzyme, and dUTP fluorophore conjugate necessary to complete the TUNEL reaction in fixed cells or tissue. This kit is intended for use with fluorescence microscopy and/or flow cytometry.
Fluorescent Properties
- Excitation maximum = 490 nm / Emission maximum = 515 nm
Specificity / Sensitivity
Tissue processing or cell degradation may result in labeling of nuclei in non-apoptotic cells.
Background
Apoptosis is a regulated cellular suicide mechanism characterized by nuclear condensation, cell shrinkage, membrane blebbing, and DNA fragmentation (1). During late stages of apoptosis, DNA is fragmented by an endonuclease that cleaves the chromatin into nucleosomal units which can be visualized on gels as DNA laddering (2). DNA fragmentation also serves as a basis for monitoring apoptosis in situ using the TUNEL assay (3). In this assay, terminal deoxynucleotidyl transferase enzymatically incorporates a fluorophore conjugated nucleotide to the 3' end of fragmented DNA. Apoptosis can be monitored by an increase in TUNEL staining within intact cells.
限制使用
除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。
专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our
Trademark Information page.