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Product last modified at: 2024-12-17T18:50:42.663Z
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PDP - Template Name: Assay Kit One Column
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Senescence β-Galactosidase Activity Assay Kit (Fluorescence, Plate-Based) #23833

Senescence β-Galactosidase Activity Assay Kit (Fluorescence, Plate-Based): Image 1
The relationship between lysate protein concentration from HeLa cells, untreated and treated with Doxorubicin #5927 (200 nM, 24 hr, then rest for 3 days in fresh growth media), and the Relative Fluorescence Units (RFU) as determined by the Senescence β-Galactosidase Activity Assay Kit (Fluorescence, Plate-Based) is shown.

To Purchase # 23833S

  • Product Includes
  • Related Products
Product IncludesQuantity (with Count)
Senescence 1X Cell Lysis Buffer #910291 x 25 ml
Senescence 2X Reaction Buffer #784941 x 6.5 ml
Senescence Stop Solution #660081 x 21 ml
SA-β-Gal Substrate #459541 x 4 mg

Product Information

Protocol

Product Description

The Senescence β-Galactosidase Activity Assay Kit (Fluorescence, Plate-Based) uses the β-gal Substrate 4-Methylumbelliferyl β-D-galactopyranoside (4-MUG) to detect Senescence-Associated beta-galactosidase (SA-β-gal) activity. Upon binding to β-gal, 4-MUG is hydrolysed to the fluorescent product 4-MU that can be measured at an excitation wavelength of 360 nm and an emission wavelength of 465 nm. Fluorescent intensity correlates with sample β-gal levels. This quantitative assay uses cellular lysates for determination of SA-β-gal activity. Each kit provides enough reagents to perform up to 100 assays in a 96-well plate format.

Background

β-galactosidase (also known as β-gal) is an essential hydrolase enzyme that catalyzes the hydrolysis of galactose-containing carbohydrates into monosaccharides. Substrates of β-galactosides include lactose, various glycoproteins, ganglioside GM1, and lactosylceramides. β-galactosidase is used widely in molecular biology; for example, isolation of recombinant bacteria during molecular cloning utilizes α-complementation of the bacterial β-galactosidase gene (lacZ) in the presence of a β-gal substrate to identify recombinant clones (1). In cell biology, Senescence-Associated beta-galactosidase (SA-β-gal), defined as β-gal activity at pH 6.0, is a widely used marker of replicative senescence. While initially thought to derive from a unique isoform of β-galactosidase expressed specifically in senescent cells (2), SA-β-gal activity was subsequently shown to result from overexpression and accumulation of β-galactosidase in endogenous lysosomes, and is not specifically required for replicative senescence (3).
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