Senescence β-Galactosidase Activity Assay Kit (Fluorescence, Flow Cytometry) #35302
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Product Description
The Senescence β-Galactosidase Activity Assay Kit (Fluorescence, Flow Cytometry) allows the quantitative measure of senescence-associated β-galactosidase activity in living cells via flow cytometry using a cell permeable fluorogenic substrate, which becomes fluorescent upon hydrolysis by β-galactosidase. The fluorescent molecule is optimally excited at 490 nm (blue laser) and peak emission occurs at 514 nm (usually FL1). Each kit contains sufficient quantities of reagents to perform up to 100 assays.
Background
Limited capacity to replicate is a defining characteristic of most normal cells and culminates in senescence, an arrested state in which the cell remains viable (1). Senescent cells are not stimulated to divide by serum or passage in culture, and senescence invokes a specific cell cycle profile that differs from most damage-induced arrest processes or contact inhibition (2). An enlarged cell size, expression of pH-dependent β-galactosidase activity (3), and an altered pattern of gene expression (4,5) further characterize senescent cells.
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- Dimri, G. et al. (1995) Proc. Natl. Acad. Sci., USA 92, 9363-7.
- Cristofalo, V. J. et al. (1998) Crit. Rev. Eukaryot Gene Expr. 8, 43-80.
- Linskens, M. H. et al. (1995) Nucleic Acid Res. 23, 3244-51.
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