Hyaluronan Complete Tissue Staining Kit (HRP) #38822
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Immunohistochemistry (Paraffin) | 1:1000 |
Storage
All components in this kit are stable in accordance with the date printed on the outer packaging label when stored at the recommended temperature.
Protocol
Product Description
The Hyaluronan Complete Tissue Staining Kit (HRP) can be used to visualize hyaluronan (HA) levels in paraffin-embedded tissue sections. The kit includes an HA binding fusion protein, VersicanG1-Fc (Rabbit IgG) #15933, which specifically binds to HA in tissues, and SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) #8114 for detection of the Fc portion of VersicanG1-Fc (Rabbit IgG). SignalStain® Boost IHC Detection Reagent (HRP, Rabbit) is a highly sensitive, one-step, polymer-based detection reagent that is specific for rabbit IgG and is compatible with all peroxidase-based substrates.
This kit detects endogenous levels of HA in human, mouse, rat, and monkey tissues; it may also be used for HA staining of tissues from other animal species.
This kit detects endogenous levels of HA in human, mouse, rat, and monkey tissues; it may also be used for HA staining of tissues from other animal species.
Background
Hyaluronan (HA) comprises a major component of the extracellular matrix (ECM) in vertebrate connective tissues and is abundant in the cartilage, skin, brain, vitreous body, umbilical cord, and synovial fluid (1). The HA polysaccharide is a non-sulfated linear glycosaminoglycan composed of repeating disaccharide units of (1,3)-β-D-N-acetyl-glucosamine (GlcNAc)-β (1,4)-D-glucuronic acid (GlcA)-β (1). HA levels are significantly increased during inflammatory conditions such as myocardial infarction, arthritis, and during transplant, as well as cancer development (2). Two forms of HA have been classified based on size: low molecular weight (LMW HA, < 200 kDa) and high molecular weight (HMW HA > 1 million Da). Despite being composed of similar subunits, LMW HA and HMW HA exhibit different biological activities (3,4). The HMW HA molecule provides tissue hygroscopicity and viscoelasticity, modulates tissue hydration and osmotic balance, and possesses anti-inflammatory, anti-proliferative, anti-angiogenic, and immunosuppressive properties. The LMW HA molecule stimulates the expression of proinflammatory chemokines to regulate tissue wound healing and promotes cancer metastasis (3,4).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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