Hyaluronan Complete Tissue Staining Kit (Alexa Fluor® 488) #53721
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Immunohistochemistry (Paraffin) | 1:1000 |
Storage
All components in this kit are stable in accordance with the date printed on the outer packaging label when stored at the recommended temperature.
Protocol
Product Description
The Hyaluronan Complete Tissue Staining Kit (Alexa Fluor® 488) can be used to visualize hyaluronan (HA) levels in paraffin-embedded tissue sections. The kit includes an HA binding fusion protein, VersicanG1-Fc (Rabbit IgG) #15933, which specifically binds to HA in tissues, and Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 for detection of the Fc portion of VersicanG1-Fc (Rabbit IgG).
This kit detects endogenous levels of HA in human, mouse, rat, and monkey tissues; it may also be used for HA staining of tissues from other animal species.
This kit detects endogenous levels of HA in human, mouse, rat, and monkey tissues; it may also be used for HA staining of tissues from other animal species.
Background
Hyaluronan (HA) comprises a major component of the extracellular matrix (ECM) in vertebrate connective tissues and is abundant in the cartilage, skin, brain, vitreous body, umbilical cord, and synovial fluid (1). The HA polysaccharide is a non-sulfated linear glycosaminoglycan composed of repeating disaccharide units of (1,3)-β-D-N-acetyl-glucosamine (GlcNAc)-β (1,4)-D-glucuronic acid (GlcA)-β (1). HA levels are significantly increased during inflammatory conditions such as myocardial infarction, arthritis, and during transplant, as well as cancer development (2). Two forms of HA have been classified based on size: low molecular weight (LMW HA, < 200 kDa) and high molecular weight (HMW HA > 1 million Da). Despite being composed of similar subunits, LMW HA and HMW HA exhibit different biological activities (3,4). The HMW HA molecule provides tissue hygroscopicity and viscoelasticity, modulates tissue hydration and osmotic balance, and possesses anti-inflammatory, anti-proliferative, anti-angiogenic, and immunosuppressive properties. The LMW HA molecule stimulates the expression of proinflammatory chemokines to regulate tissue wound healing and promotes cancer metastasis (3,4).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
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