X-34 #74193
Filter:
- IF
Product Information
Product Usage Information
Supplied Reagents:
1. X-34: Create a 5 mM stock solution of X-34 (200X) using the X-34 diluent. Create a 25 µM staining solution by further diluting the 5 mM stock solution using the X-34 diluent.
Note: X-34 diluent (40% EtOH, pH 10) is the recommended solvent for staining. A slight precipitate may be present, but will not interfere with the product's performance.
Additional Reagants (Not Supplied)
1. X-34 diluent (40% EtOH, pH 10): Combine 20 mL of EtOH in 29.5 mL of dH2O. Add 1 µL of 5M NaOH dropwise until the solution reaches pH 10. Bring the final volume of X-34 diluent to 50 mL with dH2O.
2. Differentiation buffer: 50 mM NaOH, 80% EtOH
Protocol:
1. Stain slides in 25 µM X-34 staining solution for 30 min at room temperature in the dark.
2. Rinse slides three times in dH2O.
3. Incubate for 2 min in the differentiation buffer.
4. Rinse slides three times in dH2O.
5. If desired, proceed with immunostaining.
6. Mount and image.
1. X-34: Create a 5 mM stock solution of X-34 (200X) using the X-34 diluent. Create a 25 µM staining solution by further diluting the 5 mM stock solution using the X-34 diluent.
Note: X-34 diluent (40% EtOH, pH 10) is the recommended solvent for staining. A slight precipitate may be present, but will not interfere with the product's performance.
Additional Reagants (Not Supplied)
1. X-34 diluent (40% EtOH, pH 10): Combine 20 mL of EtOH in 29.5 mL of dH2O. Add 1 µL of 5M NaOH dropwise until the solution reaches pH 10. Bring the final volume of X-34 diluent to 50 mL with dH2O.
2. Differentiation buffer: 50 mM NaOH, 80% EtOH
Protocol:
1. Stain slides in 25 µM X-34 staining solution for 30 min at room temperature in the dark.
2. Rinse slides three times in dH2O.
3. Incubate for 2 min in the differentiation buffer.
4. Rinse slides three times in dH2O.
5. If desired, proceed with immunostaining.
6. Mount and image.
Storage
Store lyophilized at 4ºC, desiccated. In lyophilized form, the product is stable for 12 months.
Fluorescent Properties
- Excitation maximum = 367 nm/Emission Maximum = 497 nm
Product Description
X-34 is a lipophilic and highly fluorescent marker used to examine pathological changes in Alzheimer’s disease (AD). This cellular dye was developed as a derivative of Congo red to be more compatible with in vivo applications. By replacing the azo groups (nitrogen-to-nitrogen double bonds) in Congo red with carbon-to-carbon double bonds and having more lipophilic salicylic acid groups, X-34 has proven to be highly fluorescent and have increased membrane permeability. X-34 detects β-sheet structures and labels amyloid plaques, neurofibrillary tangles (NFTs), neuropil threads, and vascular amyloid in AD brains (1).
| Molecular Weight | 402.4 g/mol |
| Purity | ≥ 90% |
| Molecular Formula | C24H18O6 |
| CAS | 215294-98-7 |
| Solubility | Soluble in DMSO at 2 mg/mL. |
限制使用
除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。
专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our
Trademark Information page.