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Render Timestamp: 2024-11-14T22:30:21.798Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-09-20 06:17:41.488
Product last modified at: 2024-10-29T08:00:14.609Z
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PDP - Template Name: Buffer
PDP - Template ID: *******ceb613c

Protease Inhibitor Cocktail (100X) #5871

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    Product Information

    Product Usage Information

    1. Briefly vortex the Protease Inhibitor Cocktail (100X) before use.

    2. Just prior to lysing cells, dilute the cocktail 1:100 in desired lysis buffer to obtain a 1X working concentration.

    Solutions and Reagents

    The Protease Inhibitor Cocktail (100X) is composed of a proprietary mix of AEBSF, Aprotinin, Bestatin, E64, Leupeptin, and Pepstatin A to promote broad spectrum protection against endogenous proteases. The cocktail does not contain EDTA (a metalloprotease inhibitor) which can be incompatible with some downstream applications (i.e. protein assays, 2D electrophoresis, etc.). If EDTA is desired as a protease inhibitor it can be added to the cell lysis buffer at a final working concentration of 5 mM.

    Storage

    Store the undiluted 100X cocktail at 4ºC. Do not freeze.

    Product Description

    When diluted in lysis buffer to a final concentration of 1X the Protease Inhibitor Cocktail prevents protein degradation by endogenous proteases present in whole cell extract. The 100X Protease Inhibitor Cocktail is a clear, colorless liquid.


    1X Protease Inhibitor Cocktail:

    1mM AEBSF

    800nM Aprotinin

    50μM Bestatin

    15μM E-64

    20μM Leupeptin

    10μM Pepstatin A

    Background

    In order to study specific target proteins of interest protease-mediated degradation during the generation of protein lysates is to be avoided. A loss of normal cellular control occurs during cell lysis, and endogenous proteases within the cell extract are free to degrade proteins in an uncontrolled manner. The addition of protease inhibitors to the cell lysis buffer aids in the preservation of target proteins in the cell extract.
      For Research Use Only. Not For Use In Diagnostic Procedures.
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