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Render Timestamp: 2024-10-24T19:24:38.775Z
Commit: 56767fe525c928647c8401233a175d0d607d385d
XML generation date: 2024-09-20 06:16:35.682
Product last modified at: 2024-10-11T17:00:10.282Z
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PDP - Template Name: Cellular Dyes
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  1. Products /
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  3. Propidium Iodide (PI)/RNase Staining Solution

Propidium Iodide (PI)/RNase Staining Solution #4087

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    Product Information

    Product Usage Information

    As a last step for fluorescent assays, add enough neat (undiluted) PI/RNase Staining Solution to submerge cells or samples. Incubate for 15 minutes at room temperature, protected from light before analysis.

    Formulation

    Supplied in aqueous buffered solution containing less than 0.09% sodium azide. As with all intercalating DNA dyes, take all necessary safety precautions when using.

    Storage

    Store at 4°C protected from light, do not aliquot or dilute. The solution is stable for 12 months.

    Concentration:
    PI = 10ug/ml
    RNase = 300 ug/ml

    Fluorescent Properties

    • Ex(Max):535 nm
    • Em(Max): 617 nm

    Background

    Propidium Iodide (PI) is a fluorescent dye which intercalates between bases and stains both DNA and RNA. Specific DNA staining is achieved by enzymatic removal of RNA with a ribonuclease (RNase). PI/RNase is commonly used as a nuclear stain in fluorescent microscopy and as a DNA content determinant in cell cycle analyses by flow cytometry. Cells in G2 and M phases of the cell cycle contain twice the DNA content compared to those in G0 and G1 phases. DNA content during S phase lies between these extremes. PI/RNase Staining Solution has excitation and emission maxima of 535 and 617 nm, respectively (orange to red range of the spectrum). This staining solution can be used in tandem with antibodies without binding interference and is a suitable DNA stain for multiplex assays. (1-3)

    限制使用

    除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。

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    For Research Use Only. Not For Use In Diagnostic Procedures.
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