Lambda Protein Phosphatase Kit #89726
Product Information
Product Usage Information
1. Add protein sample and H2O (if necessary) to make a total volume of 40 μL.
2. Add 5 μL of 10X Buffer for Protein MetalloPhosphatases (PMP) and 5 μL of 10 mM MnCl2 for PMP to make a total reaction volume of 50 μL.
3. Add 1 μL of Lambda Protein Phosphatase.
4. Incubate at 30°C for 30 min.
Note: The optimal incubation times and enzyme concentrations must be determined empirically for each individual substrate.
2. Add 5 μL of 10X Buffer for Protein MetalloPhosphatases (PMP) and 5 μL of 10 mM MnCl2 for PMP to make a total reaction volume of 50 μL.
3. Add 1 μL of Lambda Protein Phosphatase.
4. Incubate at 30°C for 30 min.
Note: The optimal incubation times and enzyme concentrations must be determined empirically for each individual substrate.
Storage
All components in this kit are stable for 24 months when stored at the recommended temperature. Aliquot to avoid repeated freeze/thaw cycles.
Product Description
The Lambda Protein Phosphatase Kit provides reagents that, when combined, have activity toward phosphorylated serine, threonine, and tyrosine residues. Lambda Protein Phosphatase is the 221 amino acid product of the ORF221 open reading frame on bacteriophage lambda. It is also an Mn2+ dependent protein phosphatase that has the ability to release phosphate groups from phosphorylated serine, threonine, and tyrosine residues in proteins. Lambda Protein Phosphatase has a concentration of 400,000 units per mL.
| Activity | 800,000 units per mg Unit Definition: One unit is defined as the amount of enzyme that hydrolyzes 1 nmol of p-Nitrophenyl Phosphate (50 mM) in 1 min at 30°C in a total reaction volume of 50 µL. |
Specificity / Sensitivity
The Lambda Protein Phosphatase Kit is active toward phosphorylated serine, threonine, and tyrosine residues.
Source / Purification
The Lambda Protein Phosphatase component is produced in a strain of E. coli that carries the bacteriophage lambda ORF221 open reading frame. The product is isolated using the T7 expression system.
限制使用
除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。
专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
All other trademarks are the property of their respective owners. Visit our
Trademark Information page.