Intracellular Flow Cytometry Kit (Methanol) #13593
Product Information
Reagents Not Included
1. Red blood cell lysis buffer
2. Unconjugated or conjugated primary antibody
3. Fluorochrome-conjugated secondary antibody (if applicable)
NOTE: When using any primary or secondary antibody for the first time, titrate the antibody to determine which dilution allows for the strongest specific signal with the least background for your sample.
4. Cellular dyes (e.g., live-dead discrimination, DNA dyes, etc.), optional
Storage
All components in this kit are stable for at least 12 months when stored at the recommended temperature and left unused. Formaldehyde fixative should be used within one month after opening. Upon receipt, #13616 should be stored at 4°C. Remaining components should be stored at room temperature.
Protocol
Product Description
The Intracellular Flow Cytometry Kit provides the supporting reagents needed to preserve protein states and enable antibodies to bind intracellular targets, for flow cytometric analysis of cells in suspension. This kit contains sufficient reagents for 100 individual samples when following the included protocol.
IMPORTANT: Please refer to the antibody product page to determine if it is validated for use in Flow Cytometry (F) and for information regarding appropriate antibody dilution. Some primary antibodies may require detergent permeabilization, which will be noted on the datasheet. Detergent is not included in this kit.
IMPORTANT: Please refer to the antibody product page to determine if it is validated for use in Flow Cytometry (F) and for information regarding appropriate antibody dilution. Some primary antibodies may require detergent permeabilization, which will be noted on the datasheet. Detergent is not included in this kit.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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