Human Fc Receptor Blocking Solution #58948
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Product Information
Product Usage Information
Prior to immunostaining, treat cells with Human Fc Receptor Blocking Solution for 10 min at room temperature, using 2.5 μg/1 million cells in approximately 100 μL Antibody Dilution Buffer (may be purchased as ready-to-use Flow Cytometry Antibody Dilution Buffer #13616 or prepared as a solution of 0.5% Bovine Serum Albumin in 1X Phosphate Buffered Saline). No wash step is needed before incubating cells with primary antibodies.
Storage
Supplied in PBS solution (pH 7.2). Stable at 4°C for 1 year after receipt. Avoid repeated freeze-thaw cycles. Maintain sterility. Storage at -20°C should be in a manual defrost freezer.
Product Description
Human Fc receptors cross-react with certain subclasses of IgG from species such as mouse, rabbit, and rat. When cells of interest express high levels of Fc receptor protein (for example, macrophage/monocyte lineages), pre-incubating live cells with Human Fc Receptor Blocking Solution prior to immunostaining will reduce this cross-reactivity.
Specificity / Sensitivity
Species Reactivity:
Human
Source / Purification
Modified human IgG1 was expressed in 293 cells.
Background
Human Fc Receptor Blocking Solution contains a modified form of human IgG1 that blocks human Fc receptors.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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