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Render Timestamp: 2024-12-19T20:54:03.541Z
Commit: f2d32940205a64f990b886d724ccee2c9935daff
XML generation date: 2024-10-25 15:57:08.018
Product last modified at: 2024-12-17T18:54:03.699Z
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PDP - Template Name: Cellular Dyes
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Hoechst 33342 #4082

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    Product Information

    Product Usage Information

    This product is soluble up to 50 mg/ml in deionized water or dimethylformamide yielding a clear yellow solution. Heating and/or sonication may be required. For standard fluorescence based assays, Hoechst 33342 stock solutions should be diluted in phosphate-buffered saline or mounting medium to a final working concentration of 1 μg/ml.

    Storage

    The lyophilized powder is stable for 1 year if kept below 0°C with desiccant. Aqueous solutions are stable for at least 1 month if kept in the dark between 2-8°C.

    Fluorescent Properties

    • Free dye excitation maximum = 340 nm
    • Free dye emission maximum = 510 nm
    • DNA complex excitation maximum = 355 nm
    • DNA complex emission maximum = 465 nm

    Product Description

    Hoechst 33342 (bisBenzimide H33342 trihydrochloride) is supplied as a lyophilized powder in 25 mg units. It can be used to examine cellular DNA in most fluorescent applications.
    Molecular Formula C27H28N6O 3(HCl) 3(H2O)

    Background

    Hoechst 33342 is a specific fluorescent DNA stain that binds within the minor groove of double-stranded AT-rich regions. This stain can be used on both live and fixed cells (1).
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
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