Deoxyribonuclease I, Recombinant (Animal-Free, Protease and RNase Free) #53331

Deoxyribonuclease I, Recombinant (Animal-Free, Protease and RNase Free), also known as DNase I, is isolated from bovine pancreas and produced recombinantly in Pichia pastoris. This endonuclease cleaves DNA to produce products with 5'-phospho and 3'-hydroxy ends (1). DNase I is dependent on calcium ions (Ca²⁺) and activated by magnesium ions (Mg²⁺) and manganese ions (Mn²⁺). The Ca²⁺ work to maintain the enzyme conformation and the Mg²⁺ and Mn²⁺ help to catalyze the cleavage of phosphodiester bonds (2). In the presence of Mg²⁺, DNase I cleaves each strand of dsDNA independently and at random. In the presence of Mn²⁺, DNase I cleaves each strand of dsDNA at approximately the same site. This enzyme can be used to remove genomic DNA from RNA preparations prior to RT-PCR, to degrade DNA templates after transcription reactions, and to remove unwanted DNA from samples prior to Northern blotting (3-5). DNAse is used in tissue dissociation protocols to digest any DNA that may be present due to cell damage.