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Render Timestamp: 2024-11-14T22:30:03.011Z
Commit: 3c1f305a63297e594ac8d7bb5424007d592d68be
XML generation date: 2024-09-20 06:22:35.094
Product last modified at: 2024-11-08T13:00:11.561Z
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PDP - Template Name: Buffer
PDP - Template ID: *******ceb613c

Cell Permeabilization Buffer (Triton X-100) #39487

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    Product Information

    Product Usage Information

    Centrifuge fixed cells and remove supernatant. Add Cell Permeabilization Buffer directly to fixed cells, using approximately 100 µl per 1 million cells. Incubate for 10 min at room temperature. Cells may be washed with phosphate-buffered saline (PBS) to remove Cell Permeabilization Buffer prior to antibody incubation, but it is not necessary. Note that the Triton™ X-100 detergent can reduce cell pellet adhesion, so care should be taken to retain cells when removing supernatant following centrifugation.

    Storage

    This product contains 0.05% sodium azide as a preservative and is stable for 12 months when stored at 4°C.

    Product Description

    This product is supplied as a 1X working solution for cell permeabilization in flow cytometry assays. Cell Signaling Technology recommends using this buffer prior to incubation with antibodies targeting intracellular proteins. Check the antibody product webpage to confirm that it is compatible with Triton™ X-100 permeabilization. This product contains enough material for 1,000 assays based on a 100 μl permeabilization volume.
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
    All other trademarks are the property of their respective owners. Visit our Trademark Information page.