Cell Fractionation Kit (Eco-friendly detergent) #42193
Product Information
Storage
Store at -20ºC. Upon receipt, Protease Inhibitor Cocktail (100X) #5871 should be stored at 4ºC. All other components should be stored at -20ºC.
Protocol
Product Description
The Cell Fractionation Kit is designed to provide a fast and efficient way of separating cultured cells into three distinct fractions: cytoplasmic, membrane/organelle, and nuclear/cytoskeletal. These fractions can then be analyzed by SDS-PAGE and western blotting. The kit includes enough buffer for 20 assays.
This kit uses ECOSURF SA-9, a non-toxic, biodegradable, non-ionic alkylpolyglucoside-based surfactant, in place of non-ionic surfactant Triton X-100 in the CST Cell Fractionation Kit #9038. Use of Triton X-100 use is being phased out of many laboratory settings due to concerns about toxicity and environmental impact. ECOSURF SA-9 is derived from seed oil and follows the "Design for Degradation" principle of Green Chemistry, thus providing a more environmentally safe option.ECOSURF SA-9 has comparable surfactant properties to Triton X-100, it does not react with charged molecules (proteins, nucleic acids), and it is stable over a wide pH range, making it a suitable replacement for many biological applications including cell fractionation. This kit performs equally as well as the original Triton-based kit.
This kit uses ECOSURF SA-9, a non-toxic, biodegradable, non-ionic alkylpolyglucoside-based surfactant, in place of non-ionic surfactant Triton X-100 in the CST Cell Fractionation Kit #9038. Use of Triton X-100 use is being phased out of many laboratory settings due to concerns about toxicity and environmental impact. ECOSURF SA-9 is derived from seed oil and follows the "Design for Degradation" principle of Green Chemistry, thus providing a more environmentally safe option.ECOSURF SA-9 has comparable surfactant properties to Triton X-100, it does not react with charged molecules (proteins, nucleic acids), and it is stable over a wide pH range, making it a suitable replacement for many biological applications including cell fractionation. This kit performs equally as well as the original Triton-based kit.
Background
Cellular fractionation allows for the extraction of cellular proteins into distinct compartments. This is achieved by the use of detergents that take advantage of the inherent qualities and composition of different cellular membranes (1). Cellular fractionation has been important for defining the localization of many proteins, observing the translocation of proteins, and determining protein-protein complexes, such as cytoskeletal-associated proteins (2,3). Thus, detergent-based cellular fractionation separates cellular components with greater ease and speed compared to a more laborious density centrifugation method (4).
限制使用
除非 CST 的合法授书代表以书面形式书行明确同意，否书以下条款适用于 CST、其关书方或分书商提供的书品。 任何书充本条款或与本条款不同的客书条款和条件，除非书 CST 的合法授书代表以书面形式书独接受， 否书均被拒书，并且无效。
专品专有“专供研究使用”的专专或专似的专专声明， 且未专得美国食品和专品管理局或其他外国或国内专管机专专专任何用途的批准、准专或专可。客专不得将任何专品用于任何专断或治专目的， 或以任何不符合专专声明的方式使用专品。CST 专售或专可的专品提供专作专最专用专的客专，且专用于研专用途。将专品用于专断、专防或治专目的， 或专专售（专独或作专专成）或其他商专目的而专专专品，均需要 CST 的专独专可。客专：(a) 不得专独或与其他材料专合向任何第三方出售、专可、 出借、捐专或以其他方式专专或提供任何专品，或使用专品制造任何商专专品，(b) 不得复制、修改、逆向工程、反专专、 反专专专品或以其他方式专专专专专品的基专专专或技专，或使用专品开专任何与 CST 的专品或服专专争的专品或服专， (c) 不得更改或专除专品上的任何商专、商品名称、徽专、专利或版专声明或专专，(d) 只能根据 CST 的专品专售条款和任何适用文档使用专品， (e) 专遵守客专与专品一起使用的任何第三方专品或服专的任何专可、服专条款或专似专专
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
All other trademarks are the property of their respective owners. Visit our
Trademark Information page.