0.5 M EDTA, pH 8.0 #7011

EDTA (Ethylenediaminetetraacetic acid) is a common laboratory chelating agent of divalent cations, such as Ca²⁺ and Mg²⁺. Ultrapure 0.5 M EDTA, pH 8.0 from Cell Signaling Technology contains no detectable DNase, RNase, or protease activity. The convenient 1 ml vials reduce the likelihood of contamination that can occur with larger volume containers. It is suitable for use in molecular biology or protein biochemistry applications that require the chelation of divalent metal cations.

This product is used in our SimpleChip^® chromatin immunoprecipitation (ChIP) assays to stop the metal-dependant enzymatic digestion of cross-linked DNA by micrococcal nuclease once the reaction is complete. It can be added to cell lysis buffers for use as a metalloprotease inhibitor. Working concentrations are typically 1-5 mM in this application.