R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
TRAIL (C92B9) Rabbit mAb (PE Conjugate) #37020
Filter:
- F
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Description
This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated TRAIL (C92B9) Rabbit mAb #3219.
Product Usage Information
| Application | Dilution |
|---|---|
| Flow Cytometry (Fixed/Permeabilized) | 1:50 |
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Specificity / Sensitivity
TRAIL (C92B9) Rabbit mAb (PE Conjugate) detects endogenous levels of total human TRAIL protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys60 of human TRAIL, within the extracellular region of the protein.
Background
Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL), also referred to as Apo2 ligand, first identified based on its sequence homology to TNF and Fas/Apo ligand is a member of the TNF family of cytokines and either exists as a type II membrane or soluble protein (1,2). TRAIL induces apoptosis in a variety of transformed cell lines and plays a role in anti-tumor and anti-viral immune surveillance (3). TRAIL signals via binding with death receptors DR4 (TRAIL-R1) (4) and DR5 (TRAIL-R2) (5-8) which can trigger apoptosis as well as NF-κB activation (7,9). Death domains on these receptors leads to the recruitment of a death-induced signaling complex (DISC) leading to caspase-8 and subsequent caspase-3 activation. In addition, TRAIL binds with decoy receptors DcR1 (TRAIL-R3) (6,8,10,11) and DcR2 (TRAIL-R4, TRUNDD) (12,13) which lack the functional cytoplasmic death domain antagonizing TRAIL-induced apoptosis. Osteoprotegerin (OPG) has also been identified as receptor capable of inhibiting TRAIL-induced apoptosis (14). The selectivity of soluble TRAIL at triggering apoptosis in transformed cells as compared to normal cells has led to its investigation as a potential cancer therapeutic (15,16).
- Wiley, S.R. et al. (1995) Immunity 3, 673-82.
- Pitti, R.M. et al. (1996) J Biol Chem 271, 12687-90.
- Almasan, A. and Ashkenazi, A. Cytokine Growth Factor Rev 14, 337-48.
- Pan, G. et al. (1997) Science 276, 111-3.
- Walczak, H. et al. (1997) EMBO J 16, 5386-97.
- MacFarlane, M. et al. (1997) J Biol Chem 272, 25417-20.
- Chaudhary, P.M. et al. (1997) Immunity 7, 821-30.
- Schneider, P. et al. (1997) FEBS Lett 416, 329-34.
- Shetty, S. et al. (2002) Apoptosis 7, 413-20.
- Sheridan, J.P. et al. (1997) Science 277, 818-21.
- Degli-Esposti, M.A. et al. (1997) J Exp Med 186, 1165-70.
- Pan, G. et al. (1998) FEBS Lett 424, 41-5.
- Marsters, S.A. et al. (1997) Curr Biol 7, 1003-6.
- Kelley, S.K. et al. (2001) J Pharmacol Exp Ther 299, 31-8.
- Walczak, H. et al. (1999) Nat Med 5, 157-63.
- Ashkenazi, A. et al. (1999) J Clin Invest 104, 155-62.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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U.S. Patent No. 7,429,487, foreign equivalents, and child patents deriving therefrom.
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