R Recombinant
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Tox2 (E3N3G) Rabbit mAb (Alexa Fluor® 488 Conjugate) #65874
Filter:
- IF
- F
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Description
This Cell Signaling Technology® antibody is conjugated to Alexa Fluor® 488 fluorescent dye under optimal conditions. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated Tox2 (E3N3G) Rabbit mAb #79181.
Product Usage Information
| Application | Dilution |
|---|---|
| Immunofluorescence (Immunocytochemistry) | 1:800 |
| Flow Cytometry (Fixed/Permeabilized) | 1:50 |
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Specificity / Sensitivity
Tox2 (E3N3G) Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of total Tox2 protein. This antibody does not cross-react with Tox, Tox3, or Tox4 proteins.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu343 of human Tox2 protein.
Background
Thymocyte selection-associated high mobility group box protein (Tox) is a DNA-binding nuclear factor and member of the evolutionarily conserved high-motility group (HMG)-box superfamily. Tox also defines a small subfamily of proteins that include Tox2, Tox3, and Tox4, all of which are highly conserved in vertebrate species but have unique tissue expression patterns and functions (1,2).
Tox2 is expressed by natural killer (NK) cells, T follicular helper (Tfh) cells, and exhausted CD8+ T cells (3,4). Tox2 and BCL6 play a critical role in Tfh cell development by generating a transcriptional feed-forward loop that drives the Tfh program (5). Tox2 induction plays a role in driving CD8+ T cell exhaustion and is being investigated as a therapeutic target for cancer immunotherapy (6).
Tox2 is expressed by natural killer (NK) cells, T follicular helper (Tfh) cells, and exhausted CD8+ T cells (3,4). Tox2 and BCL6 play a critical role in Tfh cell development by generating a transcriptional feed-forward loop that drives the Tfh program (5). Tox2 induction plays a role in driving CD8+ T cell exhaustion and is being investigated as a therapeutic target for cancer immunotherapy (6).
- O'Flaherty, E. and Kaye, J. (2003) BMC Genomics 4, 13.
- Aliahmad, P. et al. (2012) Curr Opin Immunol 24, 173-7.
- Vong, Q.P. et al. (2014) Blood 124, 3905-13.
- Han, J. et al. (2022) Clin Exp Immunol 208, 268-280.
- Xu, W. et al. (2019) Immunity 51, 826-839.e5.
- Seo, H. et al. (2019) Proc Natl Acad Sci USA 116, 12410-12415.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
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