TCRβ (H57-597) Hamster mAb (PerCP-Cy5.5® Conjugate) #51696
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Inquiry Info. # 51696
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Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Hamster (Armenian) IgG |
Application Key:
- F-Flow Cytometry
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Description
This Cell Signaling Technology antibody is conjugated to PerCP-Cy5.5® and tested in-house for direct flow cytometric analysis in mouse cells.
Product Usage Information
For optimal flow cytometry results, we recommend 0.125 μg of antibody per test.
| Application | Dilution |
|---|---|
| Flow Cytometry (Live) | 1:160 |
Storage
Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2. This product is stable for 6 months when stored at 4oC. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Specificity / Sensitivity
TCRβ (H57-597) Hamster mAb (PerCP-Cy5.5® Conjugate) recognizes endogenous levels of total TCRβ protein. This antibody detects an epitope within the extracellular domain.
Species Reactivity:
Mouse
Source / Purification
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Background
The αβ T Cell Receptor (TCR) is a heterodimer composed of an alpha chain and a beta chain expressed on the surface of T cells (1,2). It detects peptide antigen presented by major histocompatibility complex (MHC) molecules (1,2). Detection of cognate antigen leads to activation of the TCR signaling pathway through the CD3 signaling chains that associate with the TCR (1). The TCR chains are made up of a constant region and variable regions that get rearranged during T cell development in the thymus (3). There are two TCRβ constant genes, TRBC1 and TRBC2 (3). A population of normal T cells is polyclonal and contains a mix of TRBC1- and TRBC2-expressing T cells, while T cell malignancies are monoclonal and all express the same TCR which either contains TRBC1 or TRBC2 (4). Therefore, it is possible that T cell malignancies could be identified and targeted with reagents or therapies that can distinguish between TRBC1 and TRBC2 (4).
The H57-597 antibody is widely used as a phenotypic marker for T cells expressing the αβ TCR. This antibody does not cross-react with γδ TCR (5).
The H57-597 antibody is widely used as a phenotypic marker for T cells expressing the αβ TCR. This antibody does not cross-react with γδ TCR (5).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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