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SV40 Large T Antigen (D1E9E) Rabbit mAb (Sepharose® Bead Conjugate) #45159
Filter:
- IP
Supporting Data
| REACTIVITY | Vir |
| SENSITIVITY | Endogenous |
| MW (kDa) | 90 |
| Source/Isotype | Rabbit IgG |
Application Key:
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- Vir-Virus
Product Information
Product Description
This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated Sepharose® beads.
SV40 Large T Antigen (D1E9E) Rabbit mAb (Sepharose® Bead Conjugate) is useful for immunoprecipitation assays. The antibody is expected to exhibit the same cross-species reactivity as the unconjugated SV40 Large T Antigen (D1E9E) Rabbit mAb #15729.
SV40 Large T Antigen (D1E9E) Rabbit mAb (Sepharose® Bead Conjugate) is useful for immunoprecipitation assays. The antibody is expected to exhibit the same cross-species reactivity as the unconjugated SV40 Large T Antigen (D1E9E) Rabbit mAb #15729.
| MW (kDa) | 90 |
Product Usage Information
| Application | Dilution |
|---|---|
| Immunoprecipitation | 1:20 |
Storage
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
SV40 Large T Antigen (D1E9E) Rabbit mAb (Sepharose® Bead Conjugate) recognizes transgenic levels of total SV40 Large T Antigen protein.
Species Reactivity:
Virus
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys315 of SV40 Large T Antigen protein.
Background
Simian Virus 40 (SV40) is a small double-stranded, circular DNA tumor virus encoding three tumor antigens: large T, Small T, and 17KT. These antigens contribute to cellular transformation, an area of research where they are utilized extensively. Transformation of cells by SV40 large T antigen is mediated by its binding to the tumor suppressor proteins RB and p53 in the nucleus. Additional binding partners include p300, CBP, Cul7, IRS1, Bub1, Nbs1, and Fbw7, which also contribute to transformation (1).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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