R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
PIP4K2C (E7P3S) Rabbit mAb (Alexa Fluor® 647 Conjugate) #90092
Filter:
- IF
- F
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Description
This Cell Signaling Technology® antibody is conjugated to Alexa Fluor® 647 fluorescent dye under optimal conditions. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated PIP4K2C (E7P3S) Rabbit mAb #61407.
Product Usage Information
| Application | Dilution |
|---|---|
| Immunofluorescence (Frozen) | 1:50 - 1:100 |
| Flow Cytometry (Fixed/Permeabilized) | 1:3200 |
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Specificity / Sensitivity
PIP4K2C (E7P3S) Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of total PIP4K2C protein. This antibody does not cross-react with PIP4K2A or PIP3K2B proteins.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu321 of human PIP4K2C protein.
Background
Phosphatidylinositol-5-phosphate 4-kinases (PIP4Ks) synthesize phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P2), a key precursor in phosphoinositide signaling that directly modulates the activity of signaling proteins and cellular processes. There are two subfamilies of PIP kinases, type I and II, that generate PtdIns(4,5)P2 from distinct substrate pools. PIP4 type I kinases use PtdIns5P as a substrate, whereas PIP5 type II kinases use PtdIns4P (1,2). In mammalian cells, three isoforms of each PIP4K and PIP5K subfamily, encoded by distinct genes, have been characterized (3-7). All PIP kinases are stimulated by phosphatidic acid, extensively regulated by ARF and Rho GTPases, and inhibited by protein kinase A and PI-stimulated autophosphorylation (8).
PIP4K2C, also known as phosphatidylinositol 5-phosphate 4-kinase type-2 gamma (PI5P4Kγ), is a member of the type II PIP kinase family, and is broadly expressed with highest levels in kidney and brain tissue (9). PIP4K2C can form homodimers or heterodimers with its two other known family members, PIP4K2A and PIP4K2B (10). PIP4K2C is a regulator of mammalian target of rapamycin complex 1 (mTORC1) signaling and Pip4k2c KO mice demonstrate enhanced immune responses and develop autoimmunity (11,12).
PIP4K2C, also known as phosphatidylinositol 5-phosphate 4-kinase type-2 gamma (PI5P4Kγ), is a member of the type II PIP kinase family, and is broadly expressed with highest levels in kidney and brain tissue (9). PIP4K2C can form homodimers or heterodimers with its two other known family members, PIP4K2A and PIP4K2B (10). PIP4K2C is a regulator of mammalian target of rapamycin complex 1 (mTORC1) signaling and Pip4k2c KO mice demonstrate enhanced immune responses and develop autoimmunity (11,12).
- Rameh, L.E. et al. (1997) Nature 390, 192-6.
- Zhang, X. et al. (1997) J Biol Chem 272, 17756-61.
- Ishihara, H. et al. (1996) J Biol Chem 271, 23611-4.
- Loijens, J.C. and Anderson, R.A. (1996) J Biol Chem 271, 32937-43.
- Ishihara, H. et al. (1998) J Biol Chem 273, 8741-8.
- Itoh, T. et al (1998) J. Biol. Chem. 273, 20292-20299
- Boronenkov, I.V. et al. (1998) Mol Biol Cell 9, 3547-60.
- Oude Weernink, P.A. et al. (2004) Eur J Pharmacol 500, 87-99.
- Clarke, J.H. et al. (2008) Am J Physiol Renal Physiol 295, F1422-30.
- Ciruela, A. et al. (2000) Biochem J 346 Pt 3, 587-91.
- Mackey, A.M. et al. (2014) Sci Signal 7, ra104.
- Shim, H. et al. (2016) Proc Natl Acad Sci USA 113, 7596-601.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
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