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PIP4K2C (E7P3S) Rabbit mAb (Alexa Fluor® 488 Conjugate) #70116
Filter:
- IF
- F
Confocal immunofluorescent analysis of fixed frozen mouse cerebellum using PIP4K2C (E7P3S) Rabbit mAb (Alexa Fluor® 488 Conjugate) (green), TMEM119 (E4B9S) Mouse mAb #98778 (red), and ProLong Gold Antifade Reagent with DAPI #8961 (blue).
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Related Products
Product Information
Product Description
This Cell Signaling Technology® antibody is conjugated to Alexa Fluor® 488 fluorescent dye under optimal conditions. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated PIP4K2C (E7P3S) Rabbit mAb #61407.
Product Usage Information
| Application | Dilution |
|---|---|
| Immunofluorescence (Frozen) | 1:50 - 1:100 |
| Flow Cytometry (Fixed/Permeabilized) | 1:100 |
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Specificity / Sensitivity
PIP4K2C (E7P3S) Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of total PIP4K2C protein. This antibody does not cross-react with PIP4K2A or PIP3K2B proteins.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu321 of human PIP4K2C protein.
Background
Phosphatidylinositol-5-phosphate 4-kinases (PIP4Ks) synthesize phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P2), a key precursor in phosphoinositide signaling that directly modulates the activity of signaling proteins and cellular processes. There are two subfamilies of PIP kinases, type I and II, that generate PtdIns(4,5)P2 from distinct substrate pools. PIP4 type I kinases use PtdIns5P as a substrate, whereas PIP5 type II kinases use PtdIns4P (1,2). In mammalian cells, three isoforms of each PIP4K and PIP5K subfamily, encoded by distinct genes, have been characterized (3-7). All PIP kinases are stimulated by phosphatidic acid, extensively regulated by ARF and Rho GTPases, and inhibited by protein kinase A and PI-stimulated autophosphorylation (8).
PIP4K2C, also known as phosphatidylinositol-5-phosphate 4-kinase type-2 gamma (PI5P4Kγ), is a member of the type II PIP kinase family, and is broadly expressed with the highest levels in kidney and brain tissue (9). PIP4K2C can form a homodimer or heterodimer with its two other known family members, PIP4K2A and PIP4K2B (10). PIP4K2C is a regulator of mammalian target of rapamycin complex 1 (mTORC1) signaling, and Pip4k2c KO mice demonstrate enhanced immune responses and develop autoimmunity (11,12).
PIP4K2C, also known as phosphatidylinositol-5-phosphate 4-kinase type-2 gamma (PI5P4Kγ), is a member of the type II PIP kinase family, and is broadly expressed with the highest levels in kidney and brain tissue (9). PIP4K2C can form a homodimer or heterodimer with its two other known family members, PIP4K2A and PIP4K2B (10). PIP4K2C is a regulator of mammalian target of rapamycin complex 1 (mTORC1) signaling, and Pip4k2c KO mice demonstrate enhanced immune responses and develop autoimmunity (11,12).
- Rameh, L.E. et al. (1997) Nature 390, 192-6.
- Zhang, X. et al. (1997) J Biol Chem 272, 17756-61.
- Ishihara, H. et al. (1996) J Biol Chem 271, 23611-4.
- Loijens, J.C. and Anderson, R.A. (1996) J Biol Chem 271, 32937-43.
- Ishihara, H. et al. (1998) J Biol Chem 273, 8741-8.
- Itoh, T. et al (1998) J. Biol. Chem. 273, 20292-20299
- Boronenkov, I.V. et al. (1998) Mol Biol Cell 9, 3547-60.
- Oude Weernink, P.A. et al. (2004) Eur J Pharmacol 500, 87-99.
- Clarke, J.H. et al. (2008) Am J Physiol Renal Physiol 295, F1422-30.
- Ciruela, A. et al. (2000) Biochem J 346 Pt 3, 587-91.
- Mackey, A.M. et al. (2014) Sci Signal 7, ra104.
- Shim, H. et al. (2016) Proc Natl Acad Sci USA 113, 7596-601.
Pathways
Explore pathways related to this product.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
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