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PDP - Template Name: Monoclonal Antibody
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  1. Products /
  2. Antibody Conjugates /
  3. NK1.1/CD161 (PK136) Mouse mAb (PE Conjugate)

NK1.1/CD161 (PK136) Mouse mAb (PE Conjugate) #45378

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Flow Cytometry Image 1: NK1.1/CD161 (PK136) Mouse mAb (PE Conjugate)
Flow cytometric analysis of live mouse splenocytes using NK1.1/CD161 (PK136) Mouse mAb (PE Conjugate) (right)

compared to concentration-matched Mouse Isotype Control (PE Conjugate) (left), co-stained with CD3 (17A2) Rat mAb (APC Conjugate) #24265.

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To Purchase # 45378

Cat. #

Size

Price

Inventory

45378S
100 µg
¥2,280

无库存

Supporting Data

REACTIVITY M
SENSITIVITY Endogenous
MW (kDa)
Source/Isotype Mouse IgG2a kappa
Application Key:
  • F-Flow Cytometry 
Species Cross-Reactivity Key:
  • M-Mouse 

Product Information

Product Description

This Cell Signaling Technology antibody is conjugated to PE and tested in-house for direct flow cytometric analysis in mouse cells.

Product Usage Information

For optimal flow cytometry results, we recommend 0.5 μg of antibody per test.

Application Dilution
Flow Cytometry (Fixed/Permeabilized) 1:40
Flow Cytometry (Live) 1:40

Storage

Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.

Protocol

Specificity / Sensitivity

NK1.1/CD161 (PK136) Mouse mAb (PE Conjugate) recognizes endogenous levels of total NK1.1/CD161 protein. This antibody detects an epitope within the extracellular domain.

Species Reactivity:

Mouse

Source / Purification

This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Background

CD161/KLRB1 (Killer cell lectin-like receptor subfamily B member 1, also known as CLEC5B and NKR-P1A) is a type II transmembrane protein that is expressed on the majority of Natural Killer (NK) cells, NK T cells, and some T lymphocytes (1). CD161/KLRB1 is also expressed on Th17 cells, promotes their generation, and modulates their function (2). Engagement with its ligand lectin-like transcript 1 (LLT1) inhibits NK cell function, while LLT1 and CD161/KLRB1 interaction in the presence of a TCR signal enhances IFN-gamma production by T cells (3,4). There are several different CD161 isoforms in rodents and some function as activating receptors as well (5,6).

There is a family of Klrb1 genes in rodents (7). PK136 antibody recognizes a specific epitope on mouse KLRB1b/c, also referred to as NK1.1, and is commonly used for detection of mouse NK cells in certain mouse strains (CE, B6, NZB, C58, Ma/My, ST, SJL, FVB). However, the epitope is absent in other mouse strains (BALB/c, AKR, CBA, C3H, DBA, 129) (8).

Database Links

UniProt ID: P27814


Entrez-Gene Id: 17059


限制使用

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For Research Use Only. Not For Use In Diagnostic Procedures.
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