R Recombinant
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NK1.1/CD161 (E6Y9G) Rabbit mAb (Alexa Fluor® 488 Conjugate) #92556
Filter:
- IF
- F
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Description
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometric and immunofluorescent analysis in mouse cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated NK1.1/CD161 (E6Y9G) Rabbit mAb #39197.
Product Usage Information
| Application | Dilution |
|---|---|
| Immunofluorescence (Frozen) | 1:50 |
| Flow Cytometry (Live) | 1:50 |
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Specificity / Sensitivity
NK1.1/CD161 (E6Y9G) Rabbit mAb (Alexa Fluor® 488) recognizes endogenous levels of total NK1.1/CD161 protein from C57BL/6, SJL, and NZW mice, but not from BALB/c and 129S6 mice. Mice from other strains have not been tested. This antibody may cross-react with NK1.1/CD161b protein in some mouse strains. Non-specific staining was observed in mouse testis by immunohistochemistry.
Species Reactivity:
Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of mouse NK1.1/CD161 protein.
Background
CD161/KLRB1 (Killer cell lectin-like receptor subfamily B member 1, also known as CLEC5B and NKR-P1A) is a type II transmembrane protein that is expressed on the majority of Natural Killer (NK) cells, NK T cells, and some T lymphocytes (1). CD161/KLRB1 is also expressed on Th17 cells, promotes their generation, and modulates their function (2). Engagement with its ligand lectin-like transcript 1 (LLT1) inhibits NK cell function, while LLT1 and CD161/KLRB1 interaction in the presence of a TCR signal enhances IFN-gamma production by T cells (3,4). There are several different CD161 isoforms in rodents and some function as activating receptors as well (5,6).
There is a family of Klrb1 genes in rodents (7). PK136 antibody recognizes a specific epitope on mouse KLRB1b/c, also referred to as NK1.1, and is commonly used for detection of mouse NK cells in certain mouse strains (CE, B6, NZB, C58, Ma/My, ST, SJL, FVB). However, the epitope is absent in other mouse strains (BALB/c, AKR, CBA, C3H, DBA, 129) (8).
There is a family of Klrb1 genes in rodents (7). PK136 antibody recognizes a specific epitope on mouse KLRB1b/c, also referred to as NK1.1, and is commonly used for detection of mouse NK cells in certain mouse strains (CE, B6, NZB, C58, Ma/My, ST, SJL, FVB). However, the epitope is absent in other mouse strains (BALB/c, AKR, CBA, C3H, DBA, 129) (8).
- Lanier, L.L. et al. (1994) J Immunol 153, 2417-28.
- Bai, A. et al. (2014) J Immunol 193, 3366-77.
- Aldemir, H. et al. (2005) J Immunol 175, 7791-5.
- Rosen, D.B. et al. (2005) J Immunol 175, 7796-9.
- Carlyle, J.R. et al. (2006) J Immunol 176, 7511-24.
- Kirkham, C.L. and Carlyle, J.R. (2014) Front Immunol 5, 214.
- Kirkham, C.L. and Carlyle, J.R. (2014) Front Immunol 5, 214.
- Carlyle, J.R. et al. (2006) J Immunol 176, 7511-24.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
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