R Recombinant
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Neutrophil Elastase (E6K6Q) Rabbit mAb (Alexa Fluor® 488 Conjugate) #74173
Filter:
- F
Supporting Data
| REACTIVITY | M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- F-Flow Cytometry
Species Cross-Reactivity Key:
- M-Mouse
- R-Rat
Product Information
Product Description
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye under optimal conditions and tested in-house for direct flow cytometric analysis in mouse cells. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated Neutrophil Elastase (E6K6Q) Rabbit mAb #61928.
Product Usage Information
| Application | Dilution |
|---|---|
| Flow Cytometry (Fixed/Permeabilized) | 1:50 |
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Specificity / Sensitivity
Neutrophil Elastase (E6K6Q) Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of total neutrophil elastase protein. This antibody does not cross-react with human neutrophil elastase protein.
Species Reactivity:
Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with full-length recombinant protein specific to mouse neutrophil elastase protein.
Background
Neutrophil elastase is a hematopoietic serine protease that belongs to the chymotrypsin superfamily and plays a critical role in the innate immune function of mature neutrophils and monocytes (1,2). Neutrophil elastase is actively synthesized as an inactive zymogen in myelocytic precursor cells of the bone marrow, which then undergoes activation by limited proteolysis and sorting to primary (azurophil) storage granules of mature neutrophil granulocytes for regulated release (3,4). Research studies have shown that neutrophils play a significant role in mediating the inflammatory response through the release of neutrophil elastase, which activates pro-inflammatory cytokines and degrades components of the extracellular matrix and Gram-negative bacteria (5). Mutations in the gene encoding neutrophil elastase, ELA2, have been implicated in hematological diseases such as cyclic and severe congenital neutropenia, which is characterized by defects in promyelocyte maturation (6,7).
- Belaaouaj, A. et al. (2000) Science 289, 1185-8.
- Tkalcevic, J. et al. (2000) Immunity 12, 201-10.
- Gullberg, U. et al. (1999) J Immunol Methods 232, 201-10.
- Takahashi, H. et al. (1988) J Biol Chem 263, 2543-7.
- Adkison, A.M. et al. (2002) J Clin Invest 109, 363-71.
- Horwitz, M. et al. (1999) Nat Genet 23, 433-6.
- Bellanné-Chantelot, C. et al. (2004) Blood 103, 4119-25.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
XP is a registered trademark of Cell Signaling Technology, Inc.
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
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