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Render Timestamp:
4/4/2025, 4:27:43 AM EDT
4/4/2025, 8:27:43 AM UTC
Commit: 070ddbed3d58ff00053de84939cefdd328876779
XML generation date: 2025-03-07 13:11:45.411
Product last modified at: 2024-09-20T07:01:22.436Z
1% for the Planet 标识
PDP - Template Name: Monoclonal Antibody (Alexa Fluor Conjugate)
PDP - Template ID: *******c8ce56b

Nestin (Rat-401) Mouse mAb (Alexa Fluor® 647 Conjugate) #8489

Filter:
  • IF
Immunofluorescence Image 1: Nestin (Rat-401) Mouse mAb (Alexa Fluor® 647 Conjugate)
Confocal immunofluorescent analysis of P1 rat cerebellum (upper) and adult rat cerebellum (lower) using Nestin (Rat-401) Mouse mAb (Alexa Fluor® 647 Conjugate) (red) and Neurofilament-L (C28E10) Rabbit mAb (Alexa Fluor® 488 Conjugate) #8024 (green). Blue pseudocolor = Propidium Iodide (PI)/RNase Staining Solution #4087.
This product is discontinued

Inquiry Info. # 8489

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Supporting Data

REACTIVITY R
SENSITIVITY Endogenous
MW (kDa)
Source/Isotype Mouse IgG1
Application Key:
  • IF-Immunofluorescence 
Species Cross-Reactivity Key:
  • R-Rat 

Product Information

Product Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 647 fluorescent dye and tested in-house for immunofluorescent analysis in rat brain. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated Nestin (Rat-401) Mouse mAb #4760.

Product Usage Information

Application Dilution
Immunofluorescence (Frozen) 1:50

Storage

Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Protocol

Specificity / Sensitivity

Nestin (Rat-401) Mouse mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of nestin protein.

Species Reactivity:

Rat

Source / Purification

Monoclonal antibody is produced by immunizing animals with E15 rat spinal cord.

Background

Nestin is an intermediate filament family member protein that is structurally related to the neurofilament proteins (1). It is highly expressed in the developing brain, where it may help to regulate cell structure and intracellular processes required for neural cell division and migration (1,2). Upon maturation of the brain, nestin expression is quickly downregulated and replaced by expression of the neurofilament proteins (2). Because nestin is expressed in both mature and precursor neuronal and glial cells, as well as in the developing brain and in the brain and spinal cord following damage, nestin is widely accepted as a marker of neural stem/progenitor cells (3). Research studies have shown that expression of nestin is also found in cells from various nervous system tumors, including gliomas, neuroblastomas, and astrocytomas, and it is generally accepted as a marker for neural cancer stem cells (3). However, nestin expression has also been observed in astrocytes, retina, cardiac muscle, pancreas, and other tissues (3). Therefore, the acceptance of nestin as an exclusive marker of neural stem/progenitor cells is not unanimous.
For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
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