R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
KLF5 (E5K6H) Rabbit mAb (Alexa Fluor® 488 Conjugate) #38716
Filter:
- F
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Description
This Cell Signaling Technology® antibody is conjugated to Alexa Fluor® 488 fluorescent dye under optimal conditions. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated KLF5 (E5K6H) Rabbit mAb #40674.
Product Usage Information
| Application | Dilution |
|---|---|
| Flow Cytometry (Fixed/Permeabilized) | 1:50 |
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Specificity / Sensitivity
KLF5 (E5K6H) Rabbit mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of total KLF5 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu140 of human KLF5 protein.
Background
Kruppel-like factors (KLFs) are a family of zinc finger DNA-binding proteins that share homology to Kruppel transcription factor in Drosophila melanogaster (1). These transcription regulators play diverse roles in cell proliferation, differentiation, inflammation, and pluripotency (2). Various KLFs, including KLF5, are involved in cancer-related processes, such as epithelial-mesenchymal transition (EMT), invasion, and metastasis (3-6). In addition, studies show that KLF5 deficiency specific to renal collecting duct epithelial cells impairs cardiac stress adaptation response coordinated by the heart and kidney (7).
- Zhang, H. et al. (2007) J Biol Chem 282, 15541-9.
- Tetreault, M.P. et al. (2013) Nat Rev Cancer 13, 701-13.
- Limame, R. et al. (2014) Oncotarget 5, 29-48.
- Qin, J. et al. (2015) Nat Commun 6, 8471.
- Cho, M.S. et al. (2016) J Immunol 196, 1412-8.
- David, C.J. et al. (2016) Cell 164, 1015-30.
- Fujiu, K. et al. (2017) Nat Med 23, 611-622.
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Alexa Fluor is a registered trademark of Life Technologies Corporation.
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].
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