G4S Linker (E7O2V) Rabbit mAb (PE-Cy^®7 Conjugate) #17399

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Supporting Data

REACTIVITY	All
SENSITIVITY	Transfected Only
MW (kDa)
Source/Isotype	Rabbit IgG

Application Key:

F-Flow Cytometry

Species Cross-Reactivity Key:

All-All Species Expected

Product Information

Product Description

This Cell Signaling Technology^® antibody is conjugated to phycoerythrin in combination with cyanine 7 (PE-Cy^®7) under optimal conditions. This antibody conjugate is expected to exhibit the same reactivity as the unconjugated G4S Linker (E7O2V) Rabbit mAb #71645, which is expected to react with cell surface-expressed CARs of varying specificity containing a G4S linker within the scFv of the extracellular domain.

Product Usage Information

Application	Dilution
Flow Cytometry (Live)	1:50

Storage

Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Protocol

Specificity / Sensitivity

G4S Linker (E7O2V) Rabbit mAb (PE-Cy^®7 Conjugate) recognizes exogenously expressed levels of scFv-based CARs containing a G4S linker.

Species Reactivity:

All Species Expected

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing three Gly4Ser repeats.

Background

The poly-Glycine-Serine (G4S) linker is a type of flexible, unstructured synthetic peptide linker sequence often leveraged to connect the variable heavy (VH) domain and variable light (VL) domain of single-chain variable fragments (scFvs) and chimeric antigen receptors (CARs) that utilize an extracellular domain scFv for target antigen recognition. The linker itself consists of a core pentapeptide sequence, Gly-Gly-Gly-Gly-Ser, that is repeated and commonly found as either a 15-mer (G4S)₃ or20-mer(G4S)₄within scFv-based CARs and scFv fragments. The linker sequence length plays a role in controlling scFv stability and the noncovalent association between the VH and VL domains (1,2).

限制使用

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Cy and CyDye are registered trademarks of GE Healthcare.

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G4S Linker (E7O2V) Rabbit mAb (PE-Cy®7 Conjugate) #17399