G4S Linker (E7O2V) Rabbit mAb (Alexa Fluor^® 647 Conjugate) #69782

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Supporting Data

REACTIVITY	H All
SENSITIVITY	Transfected Only
MW (kDa)
Source/Isotype	Rabbit IgG

Application Key:

IF-Immunofluorescence
F-Flow Cytometry

Species Cross-Reactivity Key:

H-Human
All-All Species Expected

Product Information

Product Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor^® 647 fluorescent dye under optimal conditions and tested in-house for direct flow cytometric analysis in human cells. This antibody conjugate is expected to exhibit the same reactivity as the unconjugated G4S Linker (E7O2V) Rabbit mAb #71645, which is expected to react with cell surface-expressed CARs of varying specificity containing a G4S linker within the scFv of the extracellular domain.

Product Usage Information

Application	Dilution
Immunofluorescence (Immunocytochemistry)	1:50
Flow Cytometry (Live)	1:50

Storage

Supplied in PBS (pH 7.2), less than 0.1% sodium azide, and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

Protocol

Specificity / Sensitivity

G4S Linker (E7O2V) Rabbit mAb (Alexa Fluor^® 647 Conjugate) recognizes exogenously expressed levels of scFv-based CARs containing a G4S linker.

Species Reactivity:

Human, All Species Expected

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide containing three Gly4Ser repeats.

Background

The poly-Glycine-Serine (G4S) linker is a type of flexible, unstructured synthetic peptide linker sequence often leveraged to connect the variable heavy (VH) domain and variable light (VL) domain of single-chain variable fragments (scFvs) and chimeric antigen receptors (CARs) that utilize an extracellular domain scFv for target antigen recognition. The linker itself consists of a core pentapeptide sequence, Gly-Gly-Gly-Gly-Ser, that is repeated and commonly found as either a 15-mer (G4S)₃ or20-mer(G4S)₄within scFv-based CARs and scFv fragments. The linker sequence length plays a role in controlling scFv stability and the noncovalent association between the VH and VL domains (1,2).

限制使用

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For Research Use Only. Not For Use In Diagnostic Procedures.

Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.

Alexa Fluor is a registered trademark of Life Technologies Corporation.

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or [email protected].

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G4S Linker (E7O2V) Rabbit mAb (Alexa Fluor® 647 Conjugate) #69782