FLT3 (BV10A4H2) Mouse mAb (Pacific Blue™ Conjugate) #28435
Filter:
- F
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Mouse IgG1 |
Application Key:
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Description
This Cell Signaling Technology antibody is conjugated to Pacific Blue™ fluorescent dye and tested in-house for direct flow cytometric analysis in human cells.
Product Usage Information
| Application | Dilution |
|---|---|
| Flow Cytometry (Live) | 1:50 |
Storage
Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Specificity / Sensitivity
FLT3 (BV10A4H2) Mouse mAb (Pacific Blue™ Conjugate) recognizes endogenous levels of total FLT3 protein.
Species Reactivity:
Human
Source / Purification
BV10A4H2 clone is produced by immunizing animals with the leukemic cell line BV-173 and Antibody is purified from tissue culture supernatant via protein G chromatography.
Background
FMS-related tyrosine kinase 3 (FLT3, also called FLK2) is a member of the Type III receptor tyrosine kinase family, which includes c-Kit, PDGFR, and M-CSF receptors. FLT3 is expressed on early hematopoietic progenitor cells and supports growth and differentiation within the hematopoietic system (1,2). FLT3 is activated after binding with its ligand FL, which results in a cascade of tyrosine autophosphorylation and tyrosine phosphorylation of downstream substrates (3). The p85 subunit of PI3 kinase, SHP2, GRB2, and Shc have all been reported to associate with FLT3 after FL stimulation (4-6). Tyr589/591 is located in the juxtamembrane region of FLT3 and may play an important role in regulation of FLT3 tyrosine kinase activity. Somatic mutations of FLT3 consisting of internal tandem duplications (ITDs) occur in 20% of patients with acute myeloid leukemia (7).
- Shurin, M.R. et al. (1998) Cytokine Growth Factor Rev 9, 37-48.
- Naoe, T. et al. (2001) Cancer Chemother Pharmacol 48 Suppl 1, S27-30.
- Namikawa, R. et al. (1996) Stem Cells 14, 388-95.
- Beslu, N. et al. (1996) J Biol Chem 271, 20075-81.
- Zhang, S. and Broxmeyer, H.E. (2000) Biochem Biophys Res Commun 277, 195-9.
- Zhang, S. et al. (1999) J Leukoc Biol 65, 372-80.
- Mizuki, M. et al. (2000) Blood 96, 3907-14.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
Pacific Blue is a trademark of Life Technologies Corporation.
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