R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.
Dinitrophenol (D1D6) Rabbit mAb (HRP Conjugate) #58203
Filter:
- WB
Supporting Data
| REACTIVITY | All |
| SENSITIVITY | Transfected Only |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- All-All Species Expected
Product Information
Product Description
This Cell Signaling Technology antibody is conjugated to the carbohydrate groups of horseradish peroxidase (HRP) via its amine groups. The HRP conjugated antibody is expected to exhibit the same species cross-reactivity as the unconjugated Dinitrophenol (D1D6) Rabbit mAb #14681.
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:10000 |
Storage
Supplied in 136 mM NaCl, 2.6 mM KCl, 12 mM sodium phosphate (pH 7.4) dibasic, 2 mg/ml BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Protocol
Specificity / Sensitivity
Dinitrophenol (D1D6) Rabbit mAb (HRP Conjugate) recognizes dinitrophenol-conjugated molecules.
Species Reactivity:
All Species Expected
Source / Purification
Monoclonal antibody is produced by immunizing animals with KLH-conjugated dinitrophenol.
Background
The synthetic compound dinitrophenol (DNP) is a small hapten molecule that can be conjugated to proteins for use in immunoassays (1). Antibodies that recognize dinitrophenol will display strong binding affinity to DNP conjugated molecules, including nucleic acids and proteins. These DNP-conjugated compounds can be used in in situ hybridization, western, northern, and Southern blotting (1,2). Dinitrophenol is a commonly used insecticide and has been used as a treatment for weight loss (3).
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For Research Use Only. Not For Use In Diagnostic Procedures.
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