CD8α (RPA-T8) Mouse mAb (PE Conjugate) #88829
Filter:
- F
Flow cytometric analysis of live human peripheral blood mononuclear cells using CD8α (RPA-T8) Mouse mAb (PE Conjugate) (solid line) compared to concentration-matched Mouse (MOPC-21) mAb IgG1 Isotype Control (PE Conjugate) #63630 (dashed line).
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Mouse IgG1 |
Application Key:
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- Related Products
Product Information
Product Description
This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells.
Product Usage Information
| Application | Dilution |
|---|---|
| Flow Cytometry (Fixed/Permeabilized) | 1:20 |
| Flow Cytometry (Live) | 1:20 |
Storage
Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Specificity / Sensitivity
CD8α (RPA-T8) Mouse mAb (PE Conjugate) recognizes endogenous levels of total CD8α protein. This antibody detects an epitope within the extracellular domain.
Species Reactivity:
Human
Source / Purification
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Background
Cluster of Differentiation 8 (CD8) is a disulphide-linked heterodimer consisting of the unrelated α and β subunits. Each subunit is a glycoprotein composed of a single extracellular Ig-like domain, a polypeptide linker, a transmembrane part and a short cytoplasmic tail. On T cells, CD8 is the coreceptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen–Major Histocompatibility Complex (MHC). Specifically, the Ig-like domain of CD8α interacts with the α3-domain of the MHC class I molecule. CD8 ensures specificity of the TCR–antigen interaction, prolongs the contact between the T cell and the antigen presenting cell, and the α chain recruits the tyrosine kinase Lck, which is essential for T cell activation (1).
The RPA-T8 antibody is widely used as a phenotypic marker for CD8 on cytotoxic T cells and thymocytes (2,3), as well as on certain cell types that do not express the TCR, including some NK cells (4).
The RPA-T8 antibody is widely used as a phenotypic marker for CD8 on cytotoxic T cells and thymocytes (2,3), as well as on certain cell types that do not express the TCR, including some NK cells (4).
限制使用
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For Research Use Only. Not For Use In Diagnostic Procedures.
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