CD69 (H1.2F3) Hamster mAb (PE-Cy7® Conjugate) #64490
Filter:
- F
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Hamster (Armenian) IgG |
Application Key:
- F-Flow Cytometry
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Description
This Cell Signaling Technology antibody is conjugated to PE-Cy7® and tested in-house for direct flow cytometric analysis in mouse cells.
Product Usage Information
For optimal flow cytometry results, we recommend 0.25 μg of antibody per test.
| Application | Dilution |
|---|---|
| Flow Cytometry (Fixed/Permeabilized) | 1:80 |
| Flow Cytometry (Live) | 1:80 |
Storage
Supplied in 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 6 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze.
Protocol
Specificity / Sensitivity
CD69 (H1.2F3) Hamster mAb (PE-Cy7® Conjugate) recognizes endogenous levels of total CD69 protein. This antibody detects an epitope within the extracellular domain.
Species Reactivity:
Mouse
Source / Purification
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Background
CD69, also known as Leu-23, is a type II transmembrane glycoprotein that is expressed on the surface of T cells, B cells, and NK cells (1,2). This phosphorylated disulfide-linked 28 to 32-kDa homodimer is constitutively expressed on a subset of thymocytes and platelets. It also acts as an activation antigen of lymphocytes, NK cells, neutrophils, and eosinophils (1-6). Studies have shown that stimulation of the T cell receptor (TCR) increases the expression of CD69 on the cell surface. The ability to detect the level of CD69 expression after TCR activation makes CD69 an ideal indicator of T cell activation (1).
The H1.2F3 antibody is widely used as a marker for T cell activation (7).
The H1.2F3 antibody is widely used as a marker for T cell activation (7).
- Testi, R. et al. (1989) J Immunol 142, 1854-60.
- Marzio, R. et al. (1997) J Leukoc Biol 62, 349-55.
- Lanier, L.L. et al. (1988) J Exp Med 167, 1572-85.
- Testi, R. et al. (1988) J Immunol 141, 2557-63.
- Hartnell, A. et al. (1993) Immunology 80, 281-6.
- Gavioli, R. et al. (1992) Cell Immunol 142, 186-96.
- Sobel, E.S. et al. (1993) J Immunol 150, 673-82.
限制使用
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